| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We established a system of differentiation into vascular endothelial cells (EC) and vascular smooth muscle cells (VSMC) from iPS cells of patients with CINCA syndrome. Differentiated cells had discriminative markers. EC had functions of tube formation and cytokine production. We stimulated the NLRP3 inflammasome of the obtained cells with LPS and TNFα(priming signal), ATP and silica(activation signal). There was no significant difference in production of the inflammatory cytokines and adhesion molecules between the differentiated cells from mutant iPS cells and wild-type iPS cells. NLRP3 expression in EC and VSMC from iPS cells was scarce compared with primary cells such as HUVEC and HASMC. Further study is needed to reevaluate the differentiation system, and to investigate the functions of the NLRP3 inflammasome in primary cells.
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