| Project/Area Number |
15K19673
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Gunma University |
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Principal Investigator |
Yamada Kazuya 群馬大学, 医学部附属病院, 助教 (90420190)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ラミンＡ / 光老化 / 早老症 / Atypical Werner症候群 / 老化 |
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| Outline of Final Research Achievements |
The objective of this study was to elucidate the sensitivity to cell damage induced by oxidative stress or UVA in fibroblasts from Atypical Werner syndrome (AWS) patient. Using immunofluorescence staining and flow cytometry analysis, the amount of early apoptotic cells and degree of intra-cellular reactive oxygen species (ROS) generation were higher in H202-or UVA-treated AWS fibroblasts than in normal fibroblasts, suggesting that repeated UV exposure may induce premature ageing of the skin in AWS patients and that protecting against sunlight is possibly important for delaying the emergence of AWS symptoms. In addition, we demonstrated that H2O2-,or UVA-induced apoptosis and necrosis in normal and AWS fibroblasts were enhanced by farnesyltransferase inhibitor (FTI) treatment, indicating that FTI might not be useful for treating our APS/AWS patient.
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