| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Trichohyalin-like 1 (TCHHL1) protein is a novel member of the fused-type S100 protein family expressed in the basal layer of the epidermis. In the present study, we examined the function of TCHHL1 in human skin. Cell proliferation was significantly suppressed in TCHHL1-supressed normal human keratinocytes (NHKs) compared with control NHKs. Furthermore, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cells were significantly increased by the suppression of TCHHL1 expression. In TCHHL1-supressed NHKs, the phosphorylation of ERK, JNK and AKT was inhibited. We next examined the association of TCHHL1 with the proliferation of squamous cell carcinoma (SCC). The knockdown of TCHHL1 expression in the human SCC line HSC-1 significantly suppressed the cell proliferation and induced cell death just as that in NHKs. Accordingly, we hypothesized that TCHHL1 might be a novel therapeutic target in SCC.
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