| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
D-dopachrome tautomerase (D-DT), homologue of macrophage migration inhibitory factor (MIF) has been observed to have similar functions as MIF. However, the role of D-DT in tumor biology remains unknown. It was hypothesized that D-DT and MIF could represent as a potential target for therapeutic interventions in melanoma. Here, we confirmed the production and expression of D-DT in the murine and human melanoma cells by Western blot analysis and RT-PCR. D-DT was released by all melanoma cell lines in vitro. The knock-down of D-DT and MIF by siRNA showed decrease protein expression of inflammatory cytokines and rendered them more prone to apoptosis induction. These results suggest that D-DT is associated with induction of inflammatory cytokines and apoptosis factors in melanoma cells. This qualifies D-DT for further evaluation as a therapeutic target in melanoma.
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