| Project/Area Number |
15K19685
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | メラノーマ / ddPCR法 / BRAF変異率 / 部位別定量解析 / ｄｄPCR法 |
|---|
| Outline of Final Research Achievements |
11 patients who had BRAFV600E-mutated primary lesions detected by Sanger sequencing were enrolled in this study. Further, resected metastatic lesions from 9 patients were also examined. BRAFV600E-mutated rate of primary lesions analyzed by droplet digital PCR (ddPCR) was from 18.5% to 83.3%, and the mutation rate was not varied in each lesion. 3 metastases had low mutation rate as 0.02 to 1.03%, that is, mutation cells were very rare in those metastatic tumors.
These results suggest the possibility that BRAFV600E and BRAFwild melanoma cells do not exist ununiformly, but intermingle in a single melanoma tumor in various ratios.
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