Analysis of intra- and intertumoral variety of BRAF mutation in Melanoma tumor.
Project/Area Number |
15K19685
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Dermatology
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Research Institution | Shinshu University |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | メラノーマ / ddPCR法 / BRAF変異率 / 部位別定量解析 / ddPCR法 |
Outline of Final Research Achievements |
11 patients who had BRAFV600E-mutated primary lesions detected by Sanger sequencing were enrolled in this study. Further, resected metastatic lesions from 9 patients were also examined. BRAFV600E-mutated rate of primary lesions analyzed by droplet digital PCR (ddPCR) was from 18.5% to 83.3%, and the mutation rate was not varied in each lesion. 3 metastases had low mutation rate as 0.02 to 1.03%, that is, mutation cells were very rare in those metastatic tumors. These results suggest the possibility that BRAFV600E and BRAFwild melanoma cells do not exist ununiformly, but intermingle in a single melanoma tumor in various ratios.
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Report
(3 results)
Research Products
(4 results)
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[Journal Article] Clinical characteristics associated with BRAF, NRAS and KIT mutations in Japanese melanoma patients.2015
Author(s)
Sakaizawa K, Ashida A, Uchiyama A, Ito T, Fujisawa Y, Ogata D, Matsushita S, Fujii K, Fukushima S, Shibayama Y, Hatta N, Takenouchi T, Uehara J, Okuyama R, Yamazaki N, Uhara H.
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Journal Title
J Dermatol Sci.
Volume: 80
Issue: 1
Pages: 33-37
DOI
Related Report
Peer Reviewed / Open Access
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