Identifying common and distinctive epigenetic biomarkers for schizophrenia and bipolar disorder

• Project/Area Number: 15K19748
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Psychiatric science
• Research Institution: Kumamoto University (2016); Tokyo Women's Medical University (2015)
• Principal Investigator: Sugawara Hiroko 熊本大学, 医学部附属病院, 医員 (90610692)
• Co-Investigator(Renkei-kenkyūsha): IWAMOTO Kazuya 熊本大学, 大学院生命科学研究部・分子脳科学分野, 教授 (40342753) ; BUNDO Miki 熊本大学, 大学院生命科学研究部・分子脳科学分野, 准教授 (00597221)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: エピジェネティクス / DNAメチル化 / MWAS / 統合失調症 / 双極性障害

Outline of Final Research Achievements

We analyzed DNA methylation levels of the top five regions, which showed hypomethylation in methylome-wide association study in schizophrenia(SZ), using the large sample consisted of peripheral blood of bipolar disorder(BD) patients and healthy controls(CT).
Among the five regions, significant differences of DNA methylation level between BD and CT were detected in the three regions; FAM63B, TBC1D22A and intergenic region in ch16. Detailed analysis focusing on sex revealed the hypomethylation in intergenic region in ch16 in both male and female BD, and hypomethylation in FAM63B in male BD. On the other hand, we identified hypermethylation of the CpG sites in TBC1D22A in female BD.
We identified common and distinctive epigenetic alterations between BD and SZ. These findings help to understand the pathophysiology of mental disorders and may contribute to develop the epigenetic-based biomarker using peripheral blood samples.

Research Products

• [Presentation] 統合失調症と双極性障害におけるエピゲノム要因の共通性と特異性についての検討 (2016)
  • Author(s): 菅原裕子、文東美紀、池亀天平、近藤健治、池田匡志、岩田仲生、石郷岡純、笠井清登、加藤忠史、岩本和也
  • Organizer: 第38回日本生物学的精神医学会・第59回日本神経科学大会
  • Place of Presentation: 福岡
  • Year and Date: 2016-09-08
• [Presentation] 統合失調症と双極性障害におけるエピジェネティック要因の重なりについて (2016)
  • Author(s): 菅原裕子、文東美紀、池亀天平、才田晴美、近藤健治、池田匡志、岩田仲生、石郷岡純、笠井清登、加藤忠史、岩本和也
  • Organizer: 第13回日本うつ病学会総会
  • Place of Presentation: 愛知
  • Year and Date: 2016-08-05
• [Presentation] Identifying common and distinctive epigenetic alterations between bipolar disorder and schizophrenia (2016)
  • Author(s): Hiroko Sugawara, Miki Bundo, Tempei Ikegame, Harumi Saida, Kenji Kondo, Masashi Ikeda, Nakao Iwata, Jun Ishigooka, Kiyoto Kasai, Tadafumi Kato, Kazuya Iwamoto
  • Organizer: 30th CINP World Congress of Neuropsychopharmacology
  • Place of Presentation: ソウル
  • Year and Date: 2016-07-02
  • Int'l Joint Research
• [Presentation] 統合失調症と双極性障害における共通ならびに特有のエピジェネティック要因についての検討 (2016)
  • Author(s): 菅原裕子、文東美紀、池亀天平、才田美3、近藤健治、池田匡志、岩田仲生、石郷岡純、笠井清登、加藤忠史、岩本和也
  • Organizer: 第46回日本神経精神薬理学会
  • Place of Presentation: ソウル
  • Year and Date: 2016-07-02
• [Presentation] 精神疾患の診断におけるエピジェネティックバイオマーカーの有用性の検討 (2015)
  • Author(s): 菅原裕子、岩本和也
  • Organizer: 第45回日本神経精神薬理学会・第37回日本生物学的精神医学会
  • Place of Presentation: タワーホール船堀（東京都江戸川区船堀）
  • Year and Date: 2015-09-25
  • Invited