Elucidation of time course of chromosomal aberration by radiation exposure and construction of dose-response curve for low-dose radiation

• Project/Area Number: 15K19804
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Fukushima Medical University
• Principal Investigator: Abe Yu 福島県立医科大学, 医学部, 助教 (00722472)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 細胞遺伝学的線量評価 / 低線量被ばく / CT / 染色体異常 / CT検査 / 線量評価 / 医療被ばく / 低線量 / 継続的な被ばく / 経年変化

Outline of Final Research Achievements

In this study, we analyzed the dicentric chromosome and translocation frequency of peripheral blood lymphocytes before and after multiple CT examinations and constructed the dose response curve for low dose exposure focusing on 100 mGy or less.
There was no significant accumulation of chromosomal abnormalities in exposure to CT examinations at least once a year. Therefore, contribution to carcinogenesis by CT examination is considered to be small. In addition, it was suggested that the assessment of low dose exposure is possible by increasing the number of analyzed cells.

Academic Significance and Societal Importance of the Research Achievements

本研究ではCT検査を受診した患者の生体試料を用いて，1回のCT検査による被ばくでDNA損傷が誘導されるものの約1年のインターバルを挟むとDic，転座ともに染色体異常頻度の明確な蓄積は認められないことを明らかにした。これは疾患との関連性，特に発がんに寄与する割合は低いものと考えられる。また，通常の倍に当たる2,000細胞を解析することで100 mGy以下の低線量被ばくの影響も評価可能であることが示唆された。しかし，研究開始以前の段階で染色体転座の蓄積が認められ，低線量被ばくの影響が観察できなかった可能性が考えられるため，より詳細な解析には健常者の染色体異常の自然発生頻度について調査が必要である。

Research Products

• [Journal Article] Dose-response curves for analyzing of dicentric chromosomes and chromosome translocation following doses of 1,000 mGy or less based on irradiated peripheral blood samples from 5 healthy individuals. (2018)
  • Author(s): Abe Y, Yoshida MA, Fujioka K, Kurosu Y, Ujiie R, Yanagi A, Tsuyama N, Miura T, Inaba T, Kamiya K, Sakai A
  • Journal Title: Journal of Radiation Research Volume: 59 Issue: 1 Pages: 35-42
  • DOI: 10.1093/jrr/rrx052
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of induced pluripotent stem cells from normal B cells and inducing AID expression in their differentiation into hematopoietic progenitor cells. (2017)
  • Author(s): Kawamura F, Inaki M, Katafuchi A, Abe Y, Tsuyama N, Kurosu Y, Yanagi A, Higuchi M, Muto S, Yamaura T, Suzuki H, Noji H, Suzuki S, Yoshida MA, Sasatani M, Kamiya K, Onodera M, Sakai A.
  • Journal Title: Scientific reports Volume: -
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Analysis of chromosome translocation frequency after a single CT scan in adults (2016)
  • Author(s): Yu Abe, Tomisato Miura, Mitsuaki A. Yoshida, et al.
  • Journal Title: Journal of Radiation Research, Volume: 57 Issue: 3 Pages: 1-7
  • DOI: 10.1093/jrr/rrv090
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identification of low-dose responsive metabolites in X-irradiated human B lymphoblastoid cells and fibroblasts. (2015)
  • Author(s): Tsuyama N, Mizuno H, Katafuchi A, Abe Y, Kurosu Y, Yoshida M, Kamiya K, Sakai A
  • Journal Title: J Radiat Res. Volume: 56 Issue: 1 Pages: 46-58
  • DOI: 10.1093/jrr/rru078
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Increase in dicentric chromosome formation after a single CT scan in adults. (2015)
  • Author(s): Abe Y, Miura T, Yoshida MA, Ujiie R, Kurosu Y, Kato N, Katafuchi A, Tsuyama N, Ohba T, Inamasu T, Shishido F, Noji H, Ogawa K, Yokouchi H, Kanazawa K, Ishida T, Muto S, Ohsugi J, Suzuki H, Ishikawa T, Kamiya K, Sakai A.
  • Journal Title: Scinetific Reports Volume: 9 Issue: 1 Pages: 13882-13882
  • DOI: 10.1038/srep13882
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Investigation of cumulative number of chromosome aberrations due to three consecutive CT scans (2018)
  • Author(s): Yu Abe, Hideyoshi Noji, Misaki Sugai, Yumiko Kurosu, Naohiro Tsuyama, Aki Yanagi, Yukari Yanai, Takashi Ohba, Tetsuo Ishikawa, Tomisato Miura, Kenji Kamiya, Mitsuaki A. Yoshida, Akira Sakai.
  • Organizer: American Association for Cancer Research Annual Meeting 2018
  • Int'l Joint Research
• [Presentation] Construction of dose response curves for cytogenetic biodosimetry in the low dose range based on five persons (2018)
  • Author(s): Yu Abe, Mitsuaki A Yoshida, Kurumi Fujioka, Yumiko Kurosu, Risa Ujiie, Aki Yanagi, Naohiro Tsuyama, Tomisato Miura, Toshiya Inaba, Kenji Kamiya, Akira Sakai.
  • Organizer: EPR Biodose 2018
  • Int'l Joint Research
• [Presentation] Analysis of the number of chromosome aberrations induced by three consecutive CT examinations (2018)
  • Author(s): Yu Abe, Hideyoshi Noji, Misaki Sugai, Yumiko Kurosu, Naohiro Tsuyama, Aki Yanagi, Yukari Yanai, Takashi Ohba, Tetsuo Ishikawa, Tomisato Miura, Kenji Kamiya, Mitsuaki A. Yoshida, Akira Sakai
  • Organizer: 第61回日本放射線影響学会
• [Presentation] CRISPR/Cas9を用いたt(11;14)染色体転座誘発 (2018)
  • Author(s): 津山尚宏，阿部悠，柳亜希，菅井美咲，坂井晃
  • Organizer: 第61回日本放射線影響学会
• [Presentation] Investigation of cumulative number of chromosome aberrations due to three consecutive CT scans (2018)
  • Author(s): Yu Abe, Hideyoshi Noji, Misaki Sugai, Yumiko Kurosu, Naohiro Tsuyama, Aki Yanagi, Yukari Yanai, Takashi Ohba, Tetsuo Ishikawa, Tomisato Miura, Kenji Kamiya, Mitsuaki A. Yoshida, Akia Sakai.
  • Organizer: American Association for Cancer Research (AACR) Annual Meeting 2018
  • Int'l Joint Research
• [Presentation] 継続的なCT検査による染色体異常形成数の累積性の検討 (2017)
  • Author(s): 阿部 悠, 菅井美咲, 黒須由美子, 津山尚宏, 柳 亜希, 柳井祐佳里, 吉田光明, 大葉 隆, 野地秀義, 石川徹夫, 神谷研二, 坂井 晃
  • Organizer: 日本放射線影響学会第60回大会
• [Presentation] 低線量ガンマ線照射による染色体線量評価用線量応答曲線の作製 (2016)
  • Author(s): 阿部 悠, 三浦富智, 吉田光明, 氏家里紗, 黒須由美子, 柳 亜希, 津山尚宏, 川村文彦,
  • Organizer: 日本放射線影響学会第59回大会
  • Place of Presentation: 広島県広島市
  • Year and Date: 2016-10-26
• [Presentation] Analysis of chromosome translocation frequency after a single CT scan in adults (2015)
  • Author(s): Abe Y, Miura T, Yoshida M, Ujiie R, Kurosu Y, Kato N, Katafuchi A, Tsuyama N, Kawamura F, Ohba T, Inamasu T, Shishido F, Noji H, Ogawa K, Yokouchi H, Kanazawa K, Ishida T, Ohsugi J, Suzuki H, Ishikawa T, Kamiya K, Sakai A
  • Organizer: EPR BioDose Conference 2015
  • Place of Presentation: Hanover/NH, USA
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] Analyses of dicentric chromosome formation and chromosomal translocation after a single CT scan in adults (2015)
  • Author(s): Abe Y, Miura T, Yoshida M, Ujiie R, Kurosu Y, Kato N, Katafuchi A, Tsuyama N, Ohba T, Inamasu T, Shishido F, Noji H, Ogawa K, Yokouchi H, Kanazawa K, Ishida T, Ohsugi J, Suzuki H, Ishikawa T, Kamiya K, Sakai A
  • Organizer: 15th International Congress of Radiation Research
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2015-05-25
  • Int'l Joint Research
• [Remarks] 医療被ばくによる染色体への影響解析
  • URL: https://cms2.fmu.ac.jp/cms/rls/iryohibaku_chr.html
• [Remarks] 生物学的線量評価に用いる二動原体染色体と転座型染色体解析用の検量線の作成
  • URL: https://cms2.fmu.ac.jp/cms/rls/wp-content/uploads/sites/120/2017/10/6ab75285f1e9b8f02f3ad4de4ed30076.pdf