Elucidation of the truck structure of the heavy particle beam using the deletion pattern in the hprt gene
| Project/Area Number |
15K19835
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | National Institutes for Quantum and Radiological Science and Technology |
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Principal Investigator |
OBARA Maki 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 放射線障害治療研究部, 業務補助員(任非) (80736992)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 重粒子線 / 突然変異 / 間接作用 |
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| Outline of Final Research Achievements |
This study aims to investigate the contribution of the indirect action to the mutation induction using DMSO in cells irradiated with X-rays or carbon-ion beam to ascertain the profound biological effects by the heavy particle beam. The mutation induction was detected as 6-thioguanine-resistant colonies at the hprt locus and the deletion pattern of exons in the hprt gene was analyzed by the multiplex polymerase chain reaction. The contribution of the indirect action of X-rays on the mutation frequency was approximately 80%, and both contributions of low- and high-LET carbon ions were approximately 50%. The total deletion rate was approximately 60% in cells irradiated with carbon ions. When combined with DMSO in carbon ions-irradiated cells, the total deletion rate remained persistent. These results demonstrated that the indirect action of carbon ions was unlikely to exert a substantial impact on the gene deletion pattern of carbon ions-induced mutants.
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Report
(4 results)
Research Products
(6 results)
