• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of the truck structure of the heavy particle beam using the deletion pattern in the hprt gene

Research Project

Project/Area Number 15K19835
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Radiation science
Research InstitutionNational Institutes for Quantum and Radiological Science and Technology

Principal Investigator

OBARA Maki  国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 放射線障害治療研究部, 業務補助員(任非) (80736992)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywords重粒子線 / 突然変異 / 間接作用
Outline of Final Research Achievements

This study aims to investigate the contribution of the indirect action to the mutation induction using DMSO in cells irradiated with X-rays or carbon-ion beam to ascertain the profound biological effects by the heavy particle beam. The mutation induction was detected as 6-thioguanine-resistant colonies at the hprt locus and the deletion pattern of exons in the hprt gene was analyzed by the multiplex polymerase chain reaction. The contribution of the indirect action of X-rays on the mutation frequency was approximately 80%, and both contributions of low- and high-LET carbon ions were approximately 50%. The total deletion rate was approximately 60% in cells irradiated with carbon ions. When combined with DMSO in carbon ions-irradiated cells, the total deletion rate remained persistent. These results demonstrated that the indirect action of carbon ions was unlikely to exert a substantial impact on the gene deletion pattern of carbon ions-induced mutants.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2017 2016 2015

All Presentation (6 results) (of which Int'l Joint Research: 3 results)

  • [Presentation] Contribution of indirect action to high-LET radiation-induced cell lethality and mutation2017

    • Author(s)
      Maki Obara, Ryoichi Hirayama, Akiko Uzawa, Sumitaka Hasegawa
    • Organizer
      56th Annual Conference of the Particle Therapy Co-Operative Group
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 重粒子線誘発突然変異におけるフリーラジカルの影響2017

    • Author(s)
      小原麻希、鵜澤玲子、平山亮一、長谷川純崇
    • Organizer
      第60回日本放射線影響学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 高LET放射線誘発突然変異における間接作用の寄与2016

    • Author(s)
      小原 麻希, 鵜澤 玲子, 平山 亮一, 長谷川 純崇
    • Organizer
      日本放射線影響学会第59回大会
    • Place of Presentation
      JMSアステールプラザ, 広島
    • Year and Date
      2016-10-26
    • Related Report
      2016 Research-status Report
  • [Presentation] Contribution of indirect action to high LET radiation-induced mutation2016

    • Author(s)
      Maki Obara, Ryoichi Hirayama, Akiko Uzawa, Sumitaka Hasegawa
    • Organizer
      42nd Annual Meeting of the European Radiation Research Society
    • Place of Presentation
      Amsterdam, Netherlands
    • Year and Date
      2016-09-04
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 炭素線誘発突然変異におけるフリーラジカルの影響2016

    • Author(s)
      小原 麻希, 鵜澤 玲子, 平山 亮一, 長谷川 純崇
    • Organizer
      第54回日本放射線腫瘍学会生物部会学術大会
    • Place of Presentation
      I-siteなんば, 大阪
    • Year and Date
      2016-07-15
    • Related Report
      2016 Research-status Report
  • [Presentation] Contribution of indirect action to radiation-induced mutation and cell lethality2015

    • Author(s)
      Maki Obara, Akiko Uzawa, Toshiyuki Shirai, Hiroshi Tauchi, Ryoichi Hirayama
    • Organizer
      15th International Congress of Radiation Research
    • Place of Presentation
      京都
    • Year and Date
      2015-05-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi