Role of OGG1 in cardiac allograft rejection

• Project/Area Number: 15K19845
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Shinshu University
• Principal Investigator: Gomibuchi Toshihito 信州大学, 医学部附属病院, 特任研究員 (90597668)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: IL-1β / OGG1 / 心移植

Outline of Final Research Achievements

We used a murine heterotopic cardiac transplantation model using OGG1 knockout mice. There is no difference in graft survival of fully mismatched model compared to control group. Donor hearts were harvested for examination on Days 5. In OGG1 knockout group, a severe infiltration of inflammatory cells was observed in the graft and more myocardial injury was seen. The expression of IL-1β, 8OHdG and OGG1 was analyzed by immunohistochemistry.

Academic Significance and Societal Importance of the Research Achievements

これまでに、OGG1と移植免疫についての研究は無く、これまでにない独創的な計画であると考えられる。DNA修復酵素であるOGG1が移植後の拒絶反応を抑える新たな標的分子となる可能性が示唆された。