Elucidation of the pathology and establishment of the therapy of thrombotic microangiopathy after liver transplantation - Development of a new complement measurement system -

• Project/Area Number: 15K19851
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Kyoto University
• Principal Investigator: TANAKA Hirokazu 京都大学, 医学研究科, 非常勤講師 (10751224)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 肝移植 / 補体 / 肝移植後血管内皮障害 / 補体活性測定法

Outline of Final Research Achievements

In order to elucidate the pathology of thrombotic microangiopathy after liver transplantation (TMA-LTx), We focused on the complement system, which is the most sensitive and rapid innate immunity. In this study, we developed a new complement measurement system, using Multiplex Assay and Cytometric Bead Array. Regarding ADAMTS13, which is one of the parameter of TMA-LTx, we could create a highly reliable test curve, but for the other antibodies, the measurement result was unstable though sufficient antibodies were bound to the beads. There was no particular problem in the method for preparing antibody beads. It is confirmed that a complement measurement system using Multiplex can be constructed by selecting an appropriate antibody.

Research Products

• [Journal Article] Impact of Subnormothermic Machine Perfusion Preservation in Severely Steatotic Rat Livers: A Detailed Assessment in an Isolated Setting. (2017)
  • Author(s): Okamura Y, Hata K, Tanaka H, Hirao H, Kubota T, Inamoto O, Kageyama S, Tamaki I, Yermek N, Yoshikawa J, Uemoto S.
  • Journal Title: American Journal of Transplantation Volume: In Press Issue: 5 Pages: 1204-1215
  • DOI: 10.1111/ajt.14110
  • Peer Reviewed / Open Access
• [Journal Article] Impact of Donor Age on Recipient Survival in Adult-to-adult Living-donor Liver Transplantation. (2017)
  • Author(s): Kubota T, Hata K, Sozu T, Ueda Y, Hirao H, Okamura Y, Tamaki I, Yoshikawa J, Kusakabe J, Uemoto S, et al.
  • Journal Title: Annals of Surgery Volume: In Press Issue: 6 Pages: 1126-1133
  • DOI: 10.1097/sla.0000000000002194
  • Peer Reviewed
• [Presentation] Efficacy of the novel Preservation Solution, Ecosol, in a rat liver transplantation model (2017)
  • Author(s): Tanaka H, Tolba R, et al.
  • Organizer: 52nd International Meeting of the European Society for Surgical Research
  • Place of Presentation: Amsterdam
  • Year and Date: 2017-06-14
  • Int'l Joint Research
• [Presentation] Post-operative Thrombocytopenia may Determine the Fate of Adult-to-adult Living Donor Partial Liver Transplantation; Proposal of Thrombotic Microangiopathy (TMALS) Score (2015)
  • Author(s): Hirokazu TANAKA
  • Organizer: European Society for Organ Transplantation 2015
  • Place of Presentation: Brussels
  • Year and Date: 2015-09-13
  • Int'l Joint Research