| Project/Area Number |
15K19851
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
|
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 肝移植 / 補体 / 肝移植後血管内皮障害 / 補体活性測定法 |
|---|
| Outline of Final Research Achievements |
In order to elucidate the pathology of thrombotic microangiopathy after liver transplantation (TMA-LTx), We focused on the complement system, which is the most sensitive and rapid innate immunity. In this study, we developed a new complement measurement system, using Multiplex Assay and Cytometric Bead Array. Regarding ADAMTS13, which is one of the parameter of TMA-LTx, we could create a highly reliable test curve, but for the other antibodies, the measurement result was unstable though sufficient antibodies were bound to the beads. There was no particular problem in the method for preparing antibody beads. It is confirmed that a complement measurement system using Multiplex can be constructed by selecting an appropriate antibody.
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