Analysis of innate immune cells which induces development of pathogenicTh17 cells in human intestinal lamina propria

• Project/Area Number: 15K19885
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Osaka University
• Principal Investigator: Matsuno Hiroshi 大阪大学, 医学部附属病院, 医員 (30749750)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ヒト腸管粘膜固有層 / MDR1陽性Pathogenic Th17細胞 / 自然免疫担当細胞 / 炎症性腸疾患

Outline of Final Research Achievements

We identified 4 lamina propria cell subsets (CD14-CD11clow, CD14-CD11chigh, CD14+CD163low, CD14+CD163high) in the human colon. Allogenic CD4+ MDR1- effector T cells were co-cultured with these cells, and the frequency of MDR1+ pathogenicTh17 cells were analyzed by Rh123 assay. The frequency of MDR1+ pathogenicTh17 cells in Crohn’s disease was similar to that in the normal colon. We could not found the innate immune cell which induced development of pathogenicTh17 cells.