Development of novel nucleic acid drug discovery based on WNT/beta-catenin signal regulation
| Project/Area Number |
15K19890
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 大腸癌 / WNT/beta-catenin シグナル / 核酸医薬 / ドラッグデリバリーシステム / WNTシグナル / WNT/β-catシグナル |
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| Outline of Final Research Achievements |
Wnt / beta-catenin and Ras are deeply involved in carcinogenesis and cancer stem cells of colorectal cancer, and it is expected that strong antitumor effect will be obtained by suppression of both signals. In this research, we aimed to identify microRNAs that can control the Wnt / beta-catenin and Ras signals to develop novel nucleic acid therapy. We analyzed Lgr 5, one of Wnt / beta-catenin signal receptor, and identified that the downstream signal changes based on the splicing of Lgr5. In addition, TOP-Flash luciferase assay was established in normal cells and colon cancer cells, and protein expression of downstream target molecules AXin 2, MYC, and CD 44 was confirmed. We performed microRNA array to identify microRNAs that can bind to 3 'UTR and CDS.
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Report
(3 results)
Research Products
(5 results)
