Analysis of liver NK cell activation mechanisms through CXCL9/CXCR3 chemokine axis
Project/Area Number |
15K19893
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Digestive surgery
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Research Institution | Hiroshima University |
Principal Investigator |
Ohira Masahiro 広島大学, 病院(医), 病院助教 (30397947)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | NK細胞 / TRAIL / ケモカイン / 肝切除 / 肝癌 |
Outline of Final Research Achievements |
Liver resident natural killer (NK) cells express tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a critical molecule for NK cell-mediated tumor cell killing. TRAIL+ NK cells strongly and exclusively expressed CXCR3. CXCL9, a ligand of CXCR3, was significantly decreased in the liver after hepatectomy. The kinetics of hepatic CXCL9 expression resembled those of hepatic TRAIL+ NK cell proportions after hepatectomy. Among liver-resident mononuclear cells, macrophages predominantly secreted CXCL9 in response to interferon (IFN)-gamma stimulation. Although the administration of poly I:C, an inducer of IFN-gamma, increased hepatic CXCL9 levels in both CXCR3-/- and WT mice, even after hepatectomy, only WT mice recovered TRAIL expression on NK cells. Major hepatectomy remarkably reduced the proportion of TRAIL-expressing NK cells in the liver through downregulation of the CXCL9-CXCR3 axis in mice.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] CD52-Negative NK Cells Are Abundant in the Liver and Less Susceptible to Alemtuzumab Treatment.2016
Author(s)
Hotta R, Ohira M, Matsuura T, Muraoka I, Tryphonopoulos P, Fan J, Tekin A, Selvaggi G, Levi D, Ruiz P, Ricordi C, Vianna R, Ohdan H, Waldmann H, Tzakis AG, and Nishida S.
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Journal Title
PLoS One
Volume: 11
Issue: 8
Pages: e0161618-e0161618
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Intraoperative assessment of tissue oxygen saturation of the remnant stomach by near-infrared spectroscopy in two cases of pancreatectomy following gastrectomy.2016
Author(s)
Akabane S, Ohira M, Ishiyama K, Kobayashi T, Ide K, Tahara H, Kuroda S, Tanimine N, Shimizu S, Tanabe K, and Ohdan H.
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Journal Title
International journal of surgery case reports
Volume: 22
Pages: 75-78
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Intrahepatic cholangiocarcinoma coinciding with a liver metastasis from a rectal carcinoma: a case report.2016
Author(s)
Akabane S, Ohira M, Kobayashi T, Kuroda S, Tanimine N, Shimizu S, Tahara H, Ide K, Ishiyama K, Egi H, Tanabe K, Sentani K, Yasui W, and Ohdan H.
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Journal Title
Surg Case Rep
Volume: 2
Issue: 1
Pages: 94-94
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 肝移植後肝癌再発におけるNK細胞機能脆弱化のメカニズムの解明2016
Author(s)
矢野琢也 大平真裕, 中野亮介, 石山宏平, 井手健太郎, 田原裕之, 清水誠一, 佐伯吉弘, 坂井寛, 石田伸樹, 柳川泉一郎, 田中飛鳥, 尾上隆司, 田中友加, 大段秀樹.
Organizer
第52回日本移植学会総会
Place of Presentation
東京
Year and Date
2016-09-29
Related Report
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[Presentation] 肝癌再発予防のための肝切除後ケモカイン低下による肝臓内NK 細胞機能低下の解析2016
Author(s)
矢野琢也 大平真裕, 中野亮介, 石山宏平, 井手健太郎, 田原裕之, 谷峰直樹, 佐伯吉弘, 清水誠一, 坂井寛, 広瀬貴行, 柳川泉一郎, 田中飛鳥, 田口和浩, 田中友加, 田代裕尊, 大段秀樹.
Organizer
第116回日本外科学会定期学術集会
Place of Presentation
大阪
Year and Date
2016-04-14
Related Report
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