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Ex-vivo Mitomycin-C pretreatment induced lower immunogenic potential of islets via suppressing secretion of multiple chemotaxis factors.

Research Project

Project/Area Number 15K19902
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Digestive surgery
Research InstitutionFukushima Medical University

Principal Investigator

SATO Naoya  福島県立医科大学, 医学部, 助教 (90622332)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords膵島移植 / Mitomycin-C / 生着延長 / 免疫抑制 / マイクロアレイ / サイトカイン / 免疫原性低下 / Mitomycin-C
Outline of Final Research Achievements

The purpose of this study to investigate a mechanism of islet graft engraftment by ex vivo mitomycin C (MMC) pretreatment, using comprehensive gene expression analysis of MMC-treated islets. The gene expression analysis identified significant downregulation of multiple genes encoding proinflammatory mediators with chemotactic activity, including multiple chemokines and peptidases. The result was validated by quantification of their proteins in islets culture supernatants. Moreover, we showed suppression of leukocyte-chemotactic activity of MMC-treated islets in vitro.
The ex vivo pretreatment of islets with MMC can reduce the immunogenic potential and prolong the survival of islet grafts by inducing the suppression of multiple leukocyte chemotactic factors.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2016 2015

All Journal Article (1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] Mitomycin-C処置によるグラフト生着延長効果2016

    • Author(s)
      佐藤直哉、 穴澤貴行、  後藤満一
    • Journal Title

      Organ Biology

      Volume: 22 Pages: 117-122

    • Related Report
      2015 Research-status Report
  • [Presentation] Mitomycin-C処置による膵島サイトカイン分泌抑制に関与する上流制御因子の解明2016

    • Author(s)
      佐藤直哉、見城明、丸橋繁、木村隆、渡邊淳一郎、武藤亮、西間木淳
    • Organizer
      第52回日本移植学会総会
    • Place of Presentation
      グランドプリンスホテル新高輪国際館パミール(東京都港区)
    • Year and Date
      2016-09-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] Mitomycin-C処置膵島の遺伝子プロファイルの解析;生着延長における複数の免疫細胞遊走因子抑制の可能性2016

    • Author(s)
      佐藤直哉, 芳賀淳一郎, 穴澤貴行, 見城 明, 丸橋 繁, 木村 隆, 渡邊淳一郎
    • Organizer
      第71回日本消化器外科学会総会
    • Place of Presentation
      アスティとくしま(徳島県徳島市)
    • Year and Date
      2016-07-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 分離膵島のMitomycin-C前処置による生着延長メカニズム -複数の免疫細胞遊走因子の分泌抑制が もたらす局所免疫不応答-2016

    • Author(s)
      佐藤直哉、芳賀淳一郎、穴澤貴行、見城 明、丸橋 繁、木村 隆、渡邊淳一郎、後藤満一
    • Organizer
      第116回日本外科学会定期学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2016-04-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] Comprehensive gene analysis on ex-vivo Mitomycin-C treatment as a potential protocol for inducing unresponsiveness to islet engraftment -harmonised suppression of multiple chemotaxis factors2015

    • Author(s)
      Sato N, Anazawa T, Haga J, Kenjo A, Marubashi S, Kimura T, (Wada I, Mori T), Gotoh M
    • Organizer
      IPITA-IXA-CTS 2015 Joint Congress
    • Place of Presentation
      Melbourne, Australia
    • Year and Date
      2015-11-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Inhibition of cytokine secretion from islets by ex-vivo Mitomycin-c treatment2015

    • Author(s)
      佐藤直哉, 芳賀淳一郎, 穴澤貴行, 見城 明, 丸橋 繁, 木村 隆, 佐藤 哲, 渡邊淳一郎, 菊池智弘, 後藤満一
    • Organizer
      第70回日本消化器外科学会総会
    • Place of Presentation
      浜松
    • Year and Date
      2015-07-15
    • Related Report
      2015 Research-status Report
  • [Book] 臨床膵島移植の現状.2015

    • Author(s)
      佐藤直哉, 穴澤貴行, 後藤満一
    • Total Pages
      6
    • Publisher
      医学出版
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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