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Heat shock exerts anticancer effects via regulation of aquaporin

Research Project

Project/Area Number 15K19903
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Digestive surgery
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

Kosuga Toshiyuki  京都府立医科大学, 医学(系)研究科(研究院), 助教 (00457946)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsアクアポリン5 / 胃癌 / 肝癌 / 温熱刺激 / 低浸透圧刺激 / 細胞周期
Outline of Final Research Achievements

Recent studies have shown that aquaporin5 (AQP5) is expressed in various cancers, and plays a role in tumor progression. We herein examined whether heat shock exerts anticancer effects via the changes in AQP5 expression. The expression of AQP5 protein was high in Alexander cells, a human liver cancer cell line. Heat shock decreased AQP5 on cellular membranes and in the cytoplasm of Alexander cells, and suppressed cell migration/invasion and proliferation, and induced early apoptosis and G0/G1 arrest. Meanwhile, the knockdown of AQP5 using AQP5-siRNA similarly suppressed cell migration/invasion and proliferation, and induced early apoptosis and G0/G1 arrest. Cycloheximide (CHX) chase experiments revealed that heat shock accelerated the degradation of AQP5, which was rescued under CHX and the autophagy inhibitor, bafilomycin A1. In conclusion, heat shock induces anticancer effects on liver cancer via the autophagic degradation of AQP5 on the cellular membrane and in the cytoplasm.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (15 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (12 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Heat shock exerts anticancer effects on liver cancer via autophagic degradation of aquaporin 5.2017

    • Author(s)
      Kudou M, Shiozaki A, Kosuga T, Shimizu H, Ichikawa D, Konishi H, Morimura R, Komatsu S, Ikoma H, Fujiwara H, Okamoto K, Marunaka Y, Otsuji E.
    • Journal Title

      Int J Oncol

      Volume: 50 Issue: 5 Pages: 1857-1867

    • DOI

      10.3892/ijo.2017.3940

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Regulation of osmolality for cancer treatment.2017

    • Author(s)
      Shiozaki A, Ichikawa D, Kosuga T, Marunaka Y, Otsuji E.
    • Journal Title

      J Physiol Sci

      Volume: 印刷中 Issue: 3 Pages: 353-360

    • DOI

      10.1007/s12576-017-0528-x

    • NAID

      40021176358

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Inhibition of Regulatory Volume Decrease Enhances the Cytocidal Effect of Hypotonic Shock in Hepatocellular Carcinoma.2016

    • Author(s)
      Kudou M, Shiozaki A, Kosuga T, Ichikawa D, Konishi H, Morimura R, Komatsu S, Ikoma H, Fujiwara H, Okamoto K, Hosogi S, Nakahari T, Marunaka Y, Otsuji E.
    • Journal Title

      J Cancer

      Volume: 7 Issue: 11 Pages: 1524-1533

    • DOI

      10.7150/jca.15181

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Heat shock induces anticancer effects via regulation of aquaporin52016

    • Author(s)
      Kosuga T, Shiozaki A, Kudou M, Shimizu H, Ichikawa D, Komatsu S, Konishi H, Arita T, Morimura R, Ikoma H, Otsuji E
    • Organizer
      40th World Congress of the International College of Surgeons, 62nd Annual Congress of the International College of Surgeons
    • Place of Presentation
      国立京都国際会館(Kyoto, Japan)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 低浸透圧刺激による胃癌細胞へのパクリタキセル取り込みおよび殺細胞増強効果2016

    • Author(s)
      小菅敏幸、塩﨑敦、市川大輔、工藤道弘、竹本健一、小松周平、小西博貴、有田智洋、森村玲、村山康利、岡本和真、藤原斉、大辻英吾
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 肝細胞癌に対する温熱刺激のアクアポリン5制御を介した抗腫瘍効果と、その機序の解明2016

    • Author(s)
      工藤道弘、塩﨑敦、小菅敏幸、清水浩紀、有田智洋、小西博貴、森村玲、村山康利、小松周平、栗生宜明、生駒久視、中西正芳、市川大輔、藤原斉、岡本和真、大辻英吾
    • Organizer
      第116回日本外科学会定期学術集会
    • Place of Presentation
      大阪国際会議場(大阪)
    • Year and Date
      2016-04-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 肝細胞癌におけるRVD制御を介した低浸透圧殺細胞増強効果2016

    • Author(s)
      小菅敏幸、塩﨑敦、市川大輔、小松周平、小西博貴、工藤道弘、有田智洋、森村玲、村山康利、栗生宜明、生駒久視、中西正芳、岡本和真、藤原斉、大辻英吾
    • Organizer
      第116回日本外科学会定期学術集会
    • Place of Presentation
      大阪国際会議場(大阪)
    • Year and Date
      2016-04-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 胃癌細胞株における低浸透圧細胞破壊効果とカルシウム輸送制御による殺細胞効果増強の検討2016

    • Author(s)
      竹本健一、塩﨑敦、市川大輔、小松周平、有田智洋、小菅敏幸、小西博貴、森村玲、村山康利、栗生宜明、生駒久視、中西正芳、藤原斉、岡本和真、丸中良典、大辻英吾
    • Organizer
      第116回日本外科学会定期学術集会
    • Place of Presentation
      大阪国際会議場(大阪)
    • Year and Date
      2016-04-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 胃癌細胞株に対する蒸留水細胞破壊効果の検証(in vivo and in vitro study)2015

    • Author(s)
      竹本健一、塩﨑敦、市川大輔、小菅敏幸、小西博貴、小松周平、栗生宜明、生駒久視、中西正芳、藤原斉、岡本和真、丸中良典、大辻英吾
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
  • [Presentation] 低浸透圧刺激による胃癌細胞へのパクリタキセル取り込み増強効果2015

    • Author(s)
      小菅敏幸、塩﨑敦、市川大輔、飯高大介、竹本健一、小松周平、小西博貴、岡本和真、藤原斉、有田智洋、中西正芳、村山康利、大辻英吾
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
  • [Presentation] 肝細胞癌におけるregulatory volume decrease の阻害による低浸透圧殺細胞効果の増強2015

    • Author(s)
      工藤道弘、塩﨑敦、小菅敏幸、市川大輔、小松周平、小西博貴、有田智洋、森村玲、生駒久視、藤原斉、岡本和真、丸中良典、大辻英吾
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
  • [Presentation] イオン輸送体制御による新たな食道癌治療戦略2015

    • Author(s)
      塩﨑敦、市川大輔、藤原斉、小菅敏幸、小西博貴、森村玲、村山康利、小松周平、栗生宜明、生駒久視、中西正芳、岡本和真、阪倉長平、大辻英吾
    • Organizer
      日本消化器外科学会大会
    • Place of Presentation
      東京
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
  • [Presentation] 細胞生理学を応用した新たな食道癌治療戦略2015

    • Author(s)
      塩﨑敦、藤原斉、市川大輔、小菅敏幸、小西博貴、小松周平、岡本和真、岸本光夫、丸中良典、大辻英吾
    • Organizer
      日本食道学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2015-07-02
    • Related Report
      2015 Research-status Report
  • [Presentation] マウス胃癌腹膜播種モデルにおける低浸透圧療法の安全性と治療効果の検討2015

    • Author(s)
      有吉要輔、塩﨑敦、市川大輔、小菅敏幸、小西博貴、森村玲、村山康利、小松周平、栗生宜明、生駒久視、中西正芳、藤原斉、岡本和真、阪倉長平、大辻英吾
    • Organizer
      日本外科学会定期学術集会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-04-16
    • Related Report
      2015 Research-status Report
  • [Presentation] 腹腔内遊離細胞治療を目的とした大腸癌細胞株における低浸透圧細胞破壊効果とクロライドイオン輸送制御による殺細胞効果増強の検討2015

    • Author(s)
      竹本健一、塩﨑敦、市川大輔、小松周平、小菅敏幸、小西博貴、森村玲、村山康利、栗生宜明、生駒久視、中西正芳、藤原斉、岡本和真、阪倉長平、丸中良典、大辻英吾
    • Organizer
      日本外科学会定期学術集会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-04-16
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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