| Project/Area Number |
15K19919
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Osaka University |
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Principal Investigator |
Ozawa Hideto 大阪大学, 医学系研究科, 寄附講座助教 (50747699)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 先天性心疾患 / 組織工学 / 再生医療 / 心臓血管外科学 / Tissue Engineering / 脱細胞化 / 心臓弁 / 再細胞化 / 自己組織化 |
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| Outline of Final Research Achievements |
We examined the recellularization process after fresh decellularized heart valve transplantation, in large animal experiments. METHODS: Pulmonary artery valves of heart transplant recipients removed during brain death heart transplantation were decellularized with 0.5% deoxycholic acid and 0.5% dodecyl sulfate. This valve was transplanted to the pulmonary valve position of the mini pig, and histological examination was carried out 6 months after transplantation. RESULTS: Migration of CD31, Vimentin-positive cells to the graft was observed, and the presence of cells produced cytokines such as VEGF, SDF-1 were observed and we also revealed the presence of the cells which were formed of myoblast. Theses findings suggest that new engraftment of decellularized heart valve was obtained.
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