Analysis of extracellular microRNA having protective effects against ischemia-perfusion injury of organs after ischemic preconditioning

Research Project

Project/Area Number	15K19921
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Cardiovascular surgery
Research Institution	Yamaguchi University
Principal Investigator	UENO Koji 山口大学, 医学部附属病院, 助教 (30736070)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	細胞外microRNA / エクソソーム / 虚血プレコンディショニング / microRNA
Outline of Final Research Achievements	Ischemic preconditioning (IPC) has protective effects against ischemia-perfusion injury of organs. Plasma vascular endothelial growth factor (VEGF) levels were increased 24 h after rIPC compared with those in sham-operated animals. Some microRNAs targeting VEGF were downregulated in exosomes 24 h after rIPC. The expression levels of miR-762 and miR-3072-5p targeting VEGF were downregulated in femoral CD34-positive bone marrow (BM) cells 24 h after rIPC compared with those in sham-operated controls. These data suggest that rIPC transiently increases plasma VEGF levels by downregulating miR-762 and miR-3072-5p in CD34-positive BM cells, leading to protection against organ ischemia.

Report

(3 results)