Analysis of extracellular microRNA having protective effects against ischemia-perfusion injury of organs after ischemic preconditioning
| Project/Area Number |
15K19921
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Yamaguchi University |
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Principal Investigator |
UENO Koji 山口大学, 医学部附属病院, 助教 (30736070)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 細胞外microRNA / エクソソーム / 虚血プレコンディショニング / microRNA |
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| Outline of Final Research Achievements |
Ischemic preconditioning (IPC) has protective effects against ischemia-perfusion injury of organs. Plasma vascular endothelial growth factor (VEGF) levels were increased 24 h after rIPC compared with those in sham-operated animals. Some microRNAs targeting VEGF were downregulated in exosomes 24 h after rIPC. The expression levels of miR-762 and miR-3072-5p targeting VEGF were downregulated in femoral CD34-positive bone marrow (BM) cells 24 h after rIPC compared with those in sham-operated controls. These data suggest that rIPC transiently increases plasma VEGF levels by downregulating miR-762 and miR-3072-5p in CD34-positive BM cells, leading to protection against organ ischemia.
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Report
(3 results)
Research Products
(3 results)
