| Project/Area Number |
15K19925
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Saga University |
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Principal Investigator |
Mukae Yosuke 佐賀大学, 医学部, 助教 (90746570)
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| Research Collaborator |
Morita Shigeki
Furukawa Kojiro
Itoh Manabu
Noguchi Ryo
Kitsuka Takahiro
Nakayama Koichi
Arai Ken-ichi
Node Koichi
Oyama Jun-ichi
Toda Shuji
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ヒトiPS細胞由来心筋細胞 / ヒトiPS細胞由来神経前駆細胞 / スフェロイド / 収縮率 / 心筋再生 / 心不全 |
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| Outline of Final Research Achievements |
At first year, we could fabricate beating spheroids by mixing human induced pluripotent stem cell (hiPS) cell derived neural progenitors (hiPS-NPs) for hiPS cell derived cardiomyocyte. The second year, the ratio of hiPS-NPs was set at 0%, 10%, 20%, 30%, and 40% of the spheroids. These spheroids were observed and recorded. The only 40% of hiPS-NPs group stopped beating by 14 days after spheroid fabrication. The spheroids involving 30% of hiPS-NPs showed functional shortenings rather than the 40% of hiPS-NPs spheroids. These results mean that adding hiPS-NPs to cardiomyocyte can change the contractile function of cardiac spheroid. Furthermore, we demonstrated neuro-specific antibody positive structure in the spheroid which beated for two weeks by immunofluorescence study. This result indicated the possibility of differentiation of hiPS-NPs to neural fibers in the mixed spheroid.
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