Analysis of chemoresistance mechanism using next generation sequencer in malignant pleural mesothelioma
| Project/Area Number |
15K19939
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Respiratory surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Research Collaborator |
MIYATA YOSHIHIRO 広島大学, 原爆放射線医科学研究所, 准教授
OKADA MORIHITO 広島大学, 原爆放射線医科学研究所, 教授
TAKESHIMA YUKIO 広島大学, 大学院医歯薬保健学研究院 病理学研究室, 教授
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 悪性胸膜中皮腫 / 抗癌剤耐性メカニズム / 次世代シークエンサー |
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| Outline of Final Research Achievements |
In immunostaining study, DHFR expression which is folic acid metabolizing enzyme before chemotherapy correlated significantly with the effect of chemotherapy and prognosis. DNA and RNA were extracted from 27 FFPE specimens before and after chemotherapy and 50 genes were searched by Cancer hot spot panel v 2 using NGS. Results were obtained from 19 cases, and TP53, IDH2, ABL1, FBXW7, PTEN were amplified in MPM. Analyzing 12 specimens that sufficient RNA were extracted, mutations were observed in BAP1 (5 cases), NF2 (3 cases), NOTCH1, ABL1, ATM, BRCA2, CCND1, FBXW7, FGFR3, NKX2-7, PTEN, STK11, TSC1 (one each) using Oncomine Comprehensive assay. In the analysis using FFPE, the yield of DNA and RNA in biopsy material before chemotherapy was poor, and comparative analysis was difficult.
Currently, DNA and RNA are extracted from frozen specimens of MPM 18 cases before and after chemotherapy, and NGS is used to narrow down candidate genes that are highly involved in chemoresistance mechanism.
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Report
(3 results)
Research Products
(2 results)
