| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
In immunostaining study, DHFR expression which is folic acid metabolizing enzyme before chemotherapy correlated significantly with the effect of chemotherapy and prognosis. DNA and RNA were extracted from 27 FFPE specimens before and after chemotherapy and 50 genes were searched by Cancer hot spot panel v 2 using NGS. Results were obtained from 19 cases, and TP53, IDH2, ABL1, FBXW7, PTEN were amplified in MPM. Analyzing 12 specimens that sufficient RNA were extracted, mutations were observed in BAP1 (5 cases), NF2 (3 cases), NOTCH1, ABL1, ATM, BRCA2, CCND1, FBXW7, FGFR3, NKX2-7, PTEN, STK11, TSC1 (one each) using Oncomine Comprehensive assay. In the analysis using FFPE, the yield of DNA and RNA in biopsy material before chemotherapy was poor, and comparative analysis was difficult.
Currently, DNA and RNA are extracted from frozen specimens of MPM 18 cases before and after chemotherapy, and NGS is used to narrow down candidate genes that are highly involved in chemoresistance mechanism.
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