| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In this study, we have tried (1) to perform comprehensive expression analysis using microarray with RNA samples extracted from intracranial and extracranial vessels of Moyamoya disease patients, (2) to verify the relationship with RNF 213 and PENK 143-183, which was identified by cerebrospinal fluid exhaustive analysis using SELDI-TOF-MS, (3) to identify specific genes involved in the development of Moyamoya disease based on transcriptional regulatory mechanism and pathway analysis by epigenetics analysis, finally (4) o create an animal model of Moyamoya disease. It was difficult to establish a methodology for RNA extraction from microscopic specimens of intracranial and extracranial blood vessels while maintaining sufficient quality. On the other hand, we performed expression analysis using microarray for RNA contained in cerebrospinal fluid of the patients with Moyamoya disease and the others, but eventually no significant results were obtained.
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