The analysis of expression of treatment-related genes and glioma stem cells in the hypoxic region of glioblastoma.

• Project/Area Number: 15K19977
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Yokohama City University
• Principal Investigator: OHTAKE Makoto 横浜市立大学, 附属病院, 指導診療医 (70644607)
• Research Collaborator: KAWAHARA Nobutaka ; MURATA Hidetoshi ; UMEMURA Masanari
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 神経膠芽腫 / 低酸素領域 / CuATSM / 幹細胞 / MGMT遺伝子

Outline of Final Research Achievements

Glioblastoma is the most common and most aggresive tumor in adults. One of the factors of treatment resistence is the existence of hypoxic regions located in the tumor. Cu-ATSM (Cu-diacetyl-bis(N4-methylthiosemicarbazone)) was developed as a hypoxic tracer in PET. Our previous reports revealed a relationship between Cu-ATSM uptake and hypoxic marker expression. If the treatment resistent factors are mainly existed in the hypoxic regions of glioblastoma, the safe treatment targeted the hypoxic regions such as stereotactic strategy becomes possible. In this study, we took the hypoxix tissue at the surgery, and performed immunopathological examinations and gene analysis. Moreover, we examined the target therapy for Cu-ATSM high uptake regions. Now we follow up the clinical course and examine the relationship of tissue status and clinical outcome.

Research Products

• [Journal Article] Hyperthermia and chemotherapy using Fe(Salen) nanoparticles might impact glioblastoma treatment. (2017)
  • Author(s): Ohtake M, Umemura M, Sato I, Akimoto T, Oda K, Nagasako A, Kim JH, Fujita T, Yokoyama U, Nakayama T, Hoshino Y, Ishiba M, Tokura S, Hara M, Muramoto T, Yamada S, Masuda T, Aoki I, Takemura Y, Murata H, Eguchi H, Kawahara N, Ishikawa Y.
  • Journal Title: Sci Rep. Volume: 7 Issue: 1 Pages: 42783-42783
  • DOI: 10.1038/srep42783
  • Peer Reviewed / Open Access
• [Presentation] 神経膠芽腫に対する有機磁性体化合物を用いた化学温熱同時療法 (2016)
  • Author(s): 大竹 誠
  • Organizer: 第75回日本脳神経外科学術集会総会
  • Place of Presentation: 福岡国際会議場(福岡県福岡市)
  • Year and Date: 2016-09-29