The study of molecular target therapy for brain tumor stem cells

• Project/Area Number: 15K19979
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Keio University
• Principal Investigator: Kikuchi Ryogo 慶應義塾大学, 医学部(信濃町), 助教 (10594723)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 脳腫瘍幹細胞 / 悪性神経膠腫 / MIF / DEPDC1

Outline of Final Research Achievements

We analyzed the function of MIF and novel oncoantigen, DEPDC1, in malignant glioma. MIF bound directly to p53 and inhibited its function in brain tumor stem cells and glioma cells. MIF knockdown inhibited tumor progression in mouse brain tumor model. DEPDC1 promoted cell proliferation through NF-κB signaling pathway in glioma cells. DEPDC1 knockdown prolonged overall survival in mouse model using induced brain tumor stem cells.
Our data suggested that MIF and DEPDC1 were useful target molecules for the treatment of glioma.

Research Products

• [Presentation] 再発悪性神経膠腫に対するカクテルペプチドワクチン療法第１/2相臨床試験 (2016)
  • Author(s): 菊地亮吾、戸田正博、田村亮太、長島秀明、柴尾俊輔、齋藤克也、植田 良、佐々木光、吉田一成
  • Organizer: 第34回日本脳腫瘍学会学術集会
  • Place of Presentation: 甲府富士屋ホテル
  • Year and Date: 2016-12-04
• [Presentation] 悪性神経膠腫における 新規標的抗原DEPDC1の機能解析 (2016)
  • Author(s): 菊地亮吾、戸田正博、Sampetrean Oltea、佐谷秀行、吉田一成
  • Organizer: 日本脳神経外科学会第75回学術総会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2016-09-29
• [Presentation] 悪性神経膠腫に対する ペプチドワクチン療法の臨床試験および 新規標的抗原DEPDC1の解析 (2015)
  • Author(s): 菊地亮吾、戸田正博、長島秀明、柴尾俊輔、齋藤克也、植田 良、 サンペトラ オルテア、佐谷秀行、佐々木光、吉田一成
  • Organizer: 第33回日本脳腫瘍学会学術総会
  • Place of Presentation: グランドプリンスホテル京都（京都府京都市）
  • Year and Date: 2015-12-06
• [Presentation] Expression and FuncGonal Analysis of an oncoanGgen, DEPDC1, in malignant glioma (2015)
  • Author(s): Ryogo Kikuchi, Masahiro Toda, Oltea Sampetrean, Hideyuki Saya, Kazunari Yoshida
  • Organizer: 20th Annual Society for Neuro - Oncology Annual Scientific Meeting
  • Place of Presentation: San Antonio Marriott Rivercenter（United states of America）
  • Year and Date: 2015-11-19
  • Int'l Joint Research
• [Presentation] 悪性神経膠腫における 新規標的抗原DEPDC1の発現・機能解析 (2015)
  • Author(s): 菊地 亮吾, 戸田 正博, Sampetrean Oltea, 佐谷 秀行, 吉田 一成
  • Organizer: 日本脳神経外科学会 第74回学術総会
  • Place of Presentation: ロイトン札幌（北海道札幌市）
  • Year and Date: 2015-10-14