• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of culture method of human synovial mesenchymal stem cells with their multi-lineage differentiation potential sustained

Research Project

Project/Area Number 15K19987
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Otabe Koji  東京医科歯科大学, 再生医療研究センター, 助教 (70737288)

Co-Investigator(Renkei-kenkyūsha) SEKIYA Ichiro  東京医科歯科大学, 再生医療研究センター, 教授 (10345291)
MUNETA Takeshi  東京医科歯科大学, 運動器外科学, 教授 (50190864)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords滑膜 / 間葉系幹細胞 / 再生医療 / 滑膜幹細胞 / サイトカイン / 増殖 / 軟骨分化能 / 増殖能
Outline of Final Research Achievements

In this study, we screened the cytokines which effectively enhance the proliferation of synovial mesenchymal stem cells with their multi-lineage differentiation potential sustained. We concluded IL-1b, PDGF-AA, TNFa, FGF are the candidate cytokines for this purpose. We present these results in the 31st Japanese Orthopaedic Research meeting and 89th Japanese Tissue Culture Association annual meeting. We published three papers in international academic scientific journals.(Pretreatment with IL-1β enhances proliferation and chondrogenic potential of synovium-derived mesenchymal stem cells. Matsumura E et al. Cytotherapy. 2017 Feb., Platelet-derived growth factor (PDGF)-AA/AB in human serum are potential indicators of the proliferative capacity of human synovial mesenchymal stem cells. Mizuno M, Otabe K et al. Stem Cell Res Ther. 2015 Dec., TNFα promotes proliferation of human synovial MSCs while maintaining chondrogenic potential. Mikio Shioda et al. PLoS one 2017)

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2017 2016

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (6 results)

  • [Journal Article] Pretreatment with IL1β enhances proliferation and chondrogenic potential of synoviumderived mesenchymal stem cells.2017

    • Author(s)
      Matsumura E
    • Journal Title

      Cytotherapy.

      Volume: 19(2) Issue: 2 Pages: 181193-181193

    • DOI

      10.1016/j.jcyt.2016.11.004

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] TNFalpha は滑膜幹細胞の増殖を促進する2016

    • Author(s)
      塩田幹夫
    • Organizer
      第31回日本整形外科学会基礎学術集会
    • Place of Presentation
      福岡県 福岡市 福岡国際会議場
    • Year and Date
      2016-10-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] IL-1βの前投与による滑膜幹細胞の増殖・軟骨分化能の検討2016

    • Author(s)
      松村惠津子
    • Organizer
      第31回日本整形外科学会基礎学術集会
    • Place of Presentation
      福岡県 福岡市 福岡国際会議場
    • Year and Date
      2016-10-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] 滑膜幹細胞の増殖性を予測する自己血清評価法の開発2016

    • Author(s)
      水野満
    • Organizer
      日本組織培養学会 第89回大会
    • Place of Presentation
      大阪府豊中市 千里ライフサイエンスセンター
    • Year and Date
      2016-05-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Il-1 β Enhances Proliferation And Chondrogenic Potential Of Synovial MSCs2016

    • Author(s)
      松村 惠津子
    • Organizer
      Orthopaedic Research Society 2016 Annual Meeting
    • Place of Presentation
      Orlando, USA
    • Year and Date
      2016-03-05
    • Related Report
      2015 Research-status Report
  • [Presentation] Hypoxia enhances proliferation through increase of colony formation rate with chondrogenic potential in primary synovial mesenchymal stem cells2016

    • Author(s)
      大原 敏之
    • Organizer
      Orthopaedic Research Society 2016 Annual Meeting
    • Place of Presentation
      Orlando, USA
    • Year and Date
      2016-03-05
    • Related Report
      2015 Research-status Report
  • [Presentation] TNF αは滑膜幹細胞の増殖を促進する2016

    • Author(s)
      塩田 幹夫
    • Organizer
      第29回日本軟骨代謝学会
    • Place of Presentation
      広島
    • Year and Date
      2016-02-19
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi