Development of culture method of human synovial mesenchymal stem cells with their multi-lineage differentiation potential sustained

• Project/Area Number: 15K19987
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Otabe Koji 東京医科歯科大学, 再生医療研究センター, 助教 (70737288)
• Co-Investigator(Renkei-kenkyūsha): SEKIYA Ichiro 東京医科歯科大学, 再生医療研究センター, 教授 (10345291) ; MUNETA Takeshi 東京医科歯科大学, 運動器外科学, 教授 (50190864)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 滑膜 / 間葉系幹細胞 / 再生医療 / 滑膜幹細胞 / サイトカイン / 増殖 / 軟骨分化能 / 増殖能

Outline of Final Research Achievements

In this study, we screened the cytokines which effectively enhance the proliferation of synovial mesenchymal stem cells with their multi-lineage differentiation potential sustained. We concluded IL-1b, PDGF-AA, TNFa, FGF are the candidate cytokines for this purpose. We present these results in the 31st Japanese Orthopaedic Research meeting and 89th Japanese Tissue Culture Association annual meeting. We published three papers in international academic scientific journals.(Pretreatment with IL-1β enhances proliferation and chondrogenic potential of synovium-derived mesenchymal stem cells. Matsumura E et al. Cytotherapy. 2017 Feb., Platelet-derived growth factor (PDGF)-AA/AB in human serum are potential indicators of the proliferative capacity of human synovial mesenchymal stem cells. Mizuno M, Otabe K et al. Stem Cell Res Ther. 2015 Dec., TNFα promotes proliferation of human synovial MSCs while maintaining chondrogenic potential. Mikio Shioda et al. PLoS one 2017)

Research Products

• [Journal Article] Pretreatment with IL1β enhances proliferation and chondrogenic potential of synoviumderived mesenchymal stem cells. (2017)
  • Author(s): Matsumura E
  • Journal Title: Cytotherapy. Volume: 19(2) Issue: 2 Pages: 181193-181193
  • DOI: 10.1016/j.jcyt.2016.11.004
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] TNFalpha は滑膜幹細胞の増殖を促進する (2016)
  • Author(s): 塩田幹夫
  • Organizer: 第31回日本整形外科学会基礎学術集会
  • Place of Presentation: 福岡県 福岡市 福岡国際会議場
  • Year and Date: 2016-10-13
• [Presentation] IL-1βの前投与による滑膜幹細胞の増殖・軟骨分化能の検討 (2016)
  • Author(s): 松村惠津子
  • Organizer: 第31回日本整形外科学会基礎学術集会
  • Place of Presentation: 福岡県 福岡市 福岡国際会議場
  • Year and Date: 2016-10-13
• [Presentation] 滑膜幹細胞の増殖性を予測する自己血清評価法の開発 (2016)
  • Author(s): 水野満
  • Organizer: 日本組織培養学会 第89回大会
  • Place of Presentation: 大阪府豊中市 千里ライフサイエンスセンター
  • Year and Date: 2016-05-25
• [Presentation] Il-1 β Enhances Proliferation And Chondrogenic Potential Of Synovial MSCs (2016)
  • Author(s): 松村 惠津子
  • Organizer: Orthopaedic Research Society 2016 Annual Meeting
  • Place of Presentation: Orlando, USA
  • Year and Date: 2016-03-05
• [Presentation] Hypoxia enhances proliferation through increase of colony formation rate with chondrogenic potential in primary synovial mesenchymal stem cells (2016)
  • Author(s): 大原 敏之
  • Organizer: Orthopaedic Research Society 2016 Annual Meeting
  • Place of Presentation: Orlando, USA
  • Year and Date: 2016-03-05
• [Presentation] TNF αは滑膜幹細胞の増殖を促進する (2016)
  • Author(s): 塩田 幹夫
  • Organizer: 第29回日本軟骨代謝学会
  • Place of Presentation: 広島
  • Year and Date: 2016-02-19