Analysis of micro RNA related to osteosarcoma metastasis

• Project/Area Number: 15K20009
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: University of the Ryukyus
• Principal Investigator: Tome Yasunori 琉球大学, 医学(系)研究科(研究院), 准教授 (20547369)
• Research Collaborator: Kiyuna Tasuku 琉球大学, 医学研究科, 大学院生 ; Oshiro Hiromichi 琉球大学, 医学研究科, 大学院生
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: miRNA / 骨肉腫 / 肺転移

Outline of Final Research Achievements

Total RNAs including micro RNAs were extracted from parental human osteosarcoma 143B cells and highly metastatic 143B-LM4 cells. mir-143 and mir-145 were higher expressed in 143B-LM4 cells compared to 143B cells. Moreover, DNA microarray analysis was performed using extracted RNAs. TANGLM and LASP1, which are related to mir-143 and mir-145, were highly expressed. Further analysis would be performed with 143B cells and 143B-LM4 cells regarding TANGLM and LASP1.

Research Products

• [Journal Article] Comparison of “Dimensionality” of cancer cell culture with Gelfoam histoculture and Matrigel. (2018)
  • Author(s): Yasunori Tome, Fuminari Uehara, Fuminori Kanaya, and Robert M. Hoffman.
  • Journal Title: Methods Mol Biol Volume: 1760 Pages: 205-214
  • DOI: 10.1007/978-1-4939-7745-1_19
  • ISBN: 9781493977437, 9781493977451
  • Peer Reviewed
• [Journal Article] Tumor-targeting Salmonella typhimurium A1-R inhibited osteosarcoma angiogenesis in the in vivo Gelfoam R assay visualized by color-coded imaging. (2018)
  • Author(s): Tasuku Kiyuna, Yasunori Tome, Fuminari Uehara, Takashi Murakami, Yong Zhang, Ming Zhao, Fuminori Kanaya, and Robert M. Hoffman.
  • Journal Title: Anticancer Volume: 38 Pages: 159-64
  • Peer Reviewed
• [Journal Article] Analysis of stroma labeling during multiple passage of a sarcoma imagenable patien-derived orthotopic xenograft (iPDOX) in red fluorescent protein transgenic nude mice. (2017)
  • Author(s): Tasuku Kiyuna, Takashi Murakami, Yasunori Tome, Kei Kawaguchi, Kentaro Igarashi, Kentaro Miyake, Fuminaroi Kanaya, Arun Singh, Fritz C. Eliber, Robert M. Hoffman.
  • Journal Title: J Cell Biochem Volume: 118(10) Issue: 10 Pages: 3367-3371
  • DOI: 10.1002/jcb.25991
  • Peer Reviewed
• [Journal Article] Labeling the stroma of a patient-derived orthotopic xenograft (PDOX) mouse model of undifferentiated pleomorphic soft-tissue sarcoma with red fluorescent for rapid non-invasive imaging for drug screening. (2017)
  • Author(s): Tasuku Kiyuna, Takashi Murakami, Yasunori Tome, Kentaro Igarashi, Kei Kawaguchi, Tara Russel, Mark Eckardt, Joseph Crompton, Arun Singh, Nicholas Bernthal, Susan Bukata, Noah Federman, Fuminori Kanaya, Fritz C. Eliber, Robert M. Hoffman.
  • Journal Title: J Cell Biochem Volume: 118(10) Issue: 2 Pages: 361-365
  • DOI: 10.1002/jcb.25643
  • Peer Reviewed
• [Journal Article] Use of αv Integrin Linked to GFP to Image Molecular Dynamics in Trafficking Cancer-Cell Emboli (2017)
  • Author(s): Tome Y, Yano S, Sugimoto N, Mii S, Bouvet M, Tsuchiya H, Kanaya F, Hoffman RM
  • Journal Title: J Cell Biochem Volume: 118 Issue: 1 Pages: 26-30
  • DOI: 10.1002/jcb.25603
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Management of local recurrences and distant metastases of soft-tissue sarcoma. (2017)
  • Author(s): Yasunori Tome, Hiroki Maehara, Tasuku Kiyuna, Hiromichi Oshiro, and Fuminori Kanaya.
  • Organizer: 19th International Society of Limb Salvage General Meeting 2017
  • Int'l Joint Research
• [Presentation] The mid-term outcome following the frozen autograft treated with liquid nitrogen for bone and soft-tissue sarcomas. (2017)
  • Author(s): Yasunori Tome, Hiroki Maehara, Tasuku Kiyuna, Hiromichi Oshiro, and Fuminori Kanaya.
  • Organizer: 19th International Society of Limb Salvage General Meeting 2017, Kanazawa, JAPAN.
  • Int'l Joint Research
• [Presentation] ヒト骨肉腫親株と高肺転移株におけるmicroRNA143/145の発現 (2015)
  • Author(s): 當銘保則
  • Organizer: 第30回日本整形外科学会基礎学術集会
  • Place of Presentation: 富山国際会議場（ 富山県富山市 ）
  • Year and Date: 2015-10-22