The circadian clock in fractured mouse femur
| Project/Area Number |
15K20012
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Kyoto Prefectural University of Medicine |
|---|
Principal Investigator |
Minami Yoichi 京都府立医科大学, 医学(系)研究科(研究院), 助教 (40415310)
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 概日リズム / 体内時計 / 発光イメージング / 軟骨 / 骨折モデル / ATDC5細胞 |
|---|
| Outline of Final Research Achievements |
The circadian clock in the cartilage formed in bone fracture healing site was studied. For this purpose, mouse femur fracture healing model was developed using PER2::Luc knock-in mouse which harbors circadian clock reporter gene. By real-time bioluminescence imaging with organ culture technique, circadian rhythm of bioluminescence in fracture healing sites were clearly detected. Noteworthy, this PER2::Luc activity rhythm was reset by parathyroid hormone (PTH).
To understand cartilage clock in detail, in vitro assay system were established using ATDC5 cell line. By real-time bioluminescence monitoring, stably transfected molecular clock reporter, Bmal1-Luc, clearly show circadian rhythms. Furthermore, as same as cartilage clock in femur growth plate and in fracture healing sites, PTH can reset the circadian clock in ATDC5. These suggest that this assay system using ATDC5 contribute for understanding the cartilage circadian clock function.
|
Report
(3 results)
Research Products
(14 results)
-
-
-
[Journal Article] Robust Circadian Rhythm and Parathyroid Hormone-Induced Resetting during Hypertrophic Differentiation in ATDC5 Chondroprogenitor Cells2015
Author(s)
Toshihiro Hosokawa, Yoshiki Tsuchiya, Naoki Okubo, Tatsuya Kunimoto, Yoichi Minami, Hiroyoshi Fujiwara, Yasuhiro Umemura, Nobuya Koike, Toshikazu Kubo, Kazuhiro Yagita
-
Journal Title
ACTA HISTOCHEMICA ET CYTOCHEMICA
Volume: 48
Issue: 6
Pages: 165-171
DOI
NAID
ISSN
0044-5991, 1347-5800
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
[Presentation] Temperature is a time cue to cartilages2016
Author(s)
Naoki Okubo, Yoichi Minami, Hiroyoshi Fujiwara, Tatsuya Kunimoto, Toshihiro Hosokawa, Ryo Oda, Toshihiro Kubo, Kazuhiro Yagita
Organizer
62th Annual Meeting of Orthopaedic Research Society
Place of Presentation
Orland, United States
Year and Date
2016-03-07
Related Report
Int'l Joint Research
-
-
-
-
