| Project/Area Number |
15K20022
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital |
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Principal Investigator |
Tanaka Nobuho 独立行政法人国立病院機構(相模原病院臨床研究センター), 政策医療企画部, 研究員 (60530920)
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| Research Collaborator |
FUKUI Naoshi
MORI Toshihito
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | 変形性関節症 / 関節炎 / エクソソーム / 関節液 / miRNA / 滑膜 |
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| Outline of Final Research Achievements |
In year 2015, we compared concentrations of 28 proteins between 16 SFs from the progressors (P-SFs) and other 16 SFs from non-progressors (NP-SFs) using Luminex. This analysis revealed that the P-SFs contain MMP-1 and MMP-3 at significantly higher concentrations compared with the NP-SFs. In year 2016, we compared miRNA profiles between the P-SFs and NP-SFs. In results, we found that the concentrations of miR-21 and miR-150 were significantly higher in the P-SFs. We next investigated relationship in concentration between the above two miRNAs and two MMPs, and found that the concentration of miR-21 or miR-150 was inversely correlated with that of MMP-1 or MMP-3. We then investigated expression of these miRNAs and MMPs in OA cartilage and synovial tissues, and found inverse correlation between the expression of either MMP and miR-21 or miR-150 in synovium. Further analyses will be conducted in future as to the origins and relationships of these miRNAs and MMPs.
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