Investigation of mechanism of anesthetic-induced amnesia and new therapy
| Project/Area Number |
15K20045
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | Osaka University |
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Principal Investigator |
Iura Akira 大阪大学, 医学系研究科, 助教 (40467551)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | GABAA受容体 / α5サブユニット / Tonic電流 / 海馬 / 麻酔薬 |
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| Outline of Final Research Achievements |
In this study, we investigated the influence of anesthetics on GABAA receptor using electrophysiological method (slice patch clamp).
Male C57BC6 mice (8 weeks age) were sacrificed and removed hippocampus under urethane anesthesia (intraperitoneal injection 1.5-2.0 g/kg). Hippocampus was sliced to 250μm. The slices were kept in standard artificial cerebrospinal fluid ACSF saturated with 95% O2 and 5% CO2 at room temperature for at least 60 min before recording. Whole-cell patch clamp (voltage clamp) recordings were performed from pyramidal cell in CA3 region of the hippocampus slices under a microscope. Membrane potentials were held at 0 mV.
Tonic current amplitude was calculated as the difference in the holding current before and after drug application. Application of midazolam increased baseline current 10-20 pA. Application of L-655708 (50nM) decreased baseline current. It is demonstrated that L-655708 antagonize current enhancement effect of midazolam in hippocampus neuron.
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Report
(3 results)
Research Products
(3 results)
