New methods for diagnosis of male infertility

• Project/Area Number: 15K20086
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Osaka University
• Principal Investigator: FUKUHARA Shinichiro 大阪大学, 医学系研究科, 助教 (20609870)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 男性不妊

Outline of Final Research Achievements

We have been establishing the method to extract RNA and purify it from fertile men and infertile men. We found that Human CPβ3 (hCPβ3) that was one of the most important actin binding proteins localized in the neck legion of human sperm had an important role for male fertile. hCPβ3 is expressed in testis, changes its localization dynamically during spermatogenesis, and has some association with male infertility. We are investigating several micro RNAs that regulate this protein.

Research Products

• [Journal Article] ART時代の男性不妊診療【男性不妊症の診察】 (2016)
  • Author(s): 福原慎一郎、宮川 康
  • Journal Title: 臨床泌尿器科 Volume: 70 Pages: 208-213
• [Presentation] Single nucleotide polymorphisms within the novel testis-specific Haspin gene encoding a serine/threonine protein kinase in human male infertility (2017)
  • Author(s): yasushi miyagawa, tetsuji soda, norichika ueda, shinichiro fukuhara, hiroshi kiuchi, akira tsujimura, hiromitsu tanaka, norio nonomura
  • Organizer: the 32nd annual EAU Congress
  • Place of Presentation: the 32nd annual EAU Congress
  • Int'l Joint Research
• [Presentation] 男性不妊症患者の射出精子における精巣特異的アクチンキャッピングプロテインの発現解析 (2016)
  • Author(s): 惣田哲次、宮川 康、上田倫央、福原慎一郎、藤田和利、木内 寛、岡本吉夫、中西佳子、一谷有希子、吉住彩香、土屋真紀、田中宏光、野々村祝夫
  • Organizer: 男性不妊症患者の射出精子における精巣特異的アクチンキャッピングプロテインの発現解析
  • Place of Presentation: パシフィコ横浜
• [Presentation] 男性不妊症患者における精巣特異的スクシニルCoaトランスフェラーゼのSNP解析 (2016)
  • Author(s): 惣田哲次、田中宏光、上田倫央、竹澤健太郎、福原慎一郎、木内 寛、宮川 康、辻村 晃、野々村祝夫
  • Organizer: 第35回日本アンドロロジー学会学術大会
  • Place of Presentation: 前橋テルサ
• [Presentation] Human capping protein β3, a novel isoform of testis-specific actin capping protein β3 subunit; the dynamic transcriptional profiles during spermatogenesis and possible implications of male infertility (2016)
  • Author(s): tetsuji soda, yasushi miyagawa, norichika ueda, kentaro takezawa, shinichiro fukuhara, hiroshi kiuchi, hiromitsu tanaka, norio nonomura
  • Organizer: AUA annual meeting 2016
  • Place of Presentation: San Diego アメリカ
  • Int'l Joint Research
• [Presentation] A novel transcriptional factor Nkapl is a germ cell-specific suppressor of Notch signaling and may be a candidate causative gene of complete maturation arrest (2016)
  • Author(s): yasushi miyagawa, hidenobu okuda, tetsuji soda, kentaro takezawa, shinichiro fukuhara, hiroshi kiuchi, akira tsujimura, masato igawa, hiromitsu tanaka, norio nonomura
  • Organizer: AUA annual meeting 2016
  • Place of Presentation: san diego アメリカ
  • Int'l Joint Research