Analysis of the mechanism for control of proliferation of ovarian and endometrial cancer stem cells

• Project/Area Number: 15K20132
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Niigata University
• Principal Investigator: Ishiguro Tatsuya 新潟大学, 医歯学総合病院, 助教 (80625690)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: がん幹細胞 / 子宮体がん / 卵巣がん / SOX2

Outline of Final Research Achievements

We　established and grew spheroid　cell cultures　from　a variety　of endometrial　and　ovarian　cancer specimens.　Endometrial cancer spheroid cells that expressed the cancer stem cell specific marker X formed larger tumors and grew more rapidly than the cells that did not express marker X. These results demonstrate the importance of marker X in proliferation of endometrial cancer stem cells. All procedures were performed following protocols approved by the Ethics and Animal Care and Use Committees of Niigata University.

Research Products

• [Journal Article] Sox2-dependent inhibition of p21 is associated with poor prognosis of endometrial cancer. (2017)
  • Author(s): Yamawaki K, Ishiguro T, Mori Y, Yoshihara K, Suda K, Tamura R, Yamaguchi M, Sekine M, Kashima K, Higuchi M, Fujii M, Okamoto K, Enomoto T
  • Journal Title: Cancer Sci. Volume: 108 Issue: 4 Pages: 632-640
  • DOI: 10.1111/cas.13196
  • Peer Reviewed / Open Access
• [Journal Article] Tumor-derived spheroids: Relevance to cancer stem cells and clinical applications. (2017)
  • Author(s): Ishiguro T, Ohata H, Sato A, Yamawaki K, Enomoto T, Okamoto K.
  • Journal Title: Cancer Sci. Volume: 108 Issue: 3 Pages: 283-289
  • DOI: 10.1111/cas.13155
  • Peer Reviewed / Open Access
• [Presentation] 婦人科悪性腫瘍がん幹細胞の特性解明 (2016)
  • Author(s): 石黒竜也
  • Organizer: 細胞凝集研究会
  • Place of Presentation: 札幌ANAホテル（北海道札幌市）
• [Presentation] 婦人科悪性腫瘍がん幹細胞の特性解明 (2015)
  • Author(s): 石黒竜也
  • Organizer: 日本産婦人科医会
  • Place of Presentation: 新潟グランドホテル （新潟県新潟市）
  • Year and Date: 2015-10-18
• [Presentation] 婦人科悪性腫瘍がん幹細胞の特性解明 (2015)
  • Author(s): 石黒竜也
  • Organizer: 新潟シンポジウム
  • Place of Presentation: 有壬記念会館 （新潟県新潟市）
  • Year and Date: 2015-08-29