Analyses of tumor heterogeneity and peritoneal tumor microenvironment in ovarian cancer for development of novel tumor immunotherapy
Project/Area Number |
15K20138
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Obstetrics and gynecology
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Research Institution | Nagoya University |
Principal Investigator |
SUZUKI Shiro 名古屋大学, 医学部附属病院, 講師 (20612758)
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Research Collaborator |
NAKATSURA Tetsuya 国立がん研究センター, 先端医療開発センター, 免疫療法開発分野長
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | 卵巣がん / 腹膜播種 |
Outline of Final Research Achievements |
We established a subclone of ID8 mouse ovarian cancer cell line (ID8-T6) which more quickly forms peritoneal dissemination. IL-33 was up-regulated in both ID8-T6 cells and tissue compared with for ID8. We confirmed the expression of IL-33 in human epithelial ovarian cancer (EOC) cell lines. Unexpectedly, the survival rate of IL33-overexpressing subclone (ID8-IL33) tumor-bearing mice was significantly prolonged compared with that of ID8-mock tumor-bearing mice. Infiltrating CD4+ cells and CD8+ cells within the disseminated tumor tissues were significantly increased in ID8-IL33 tumors compared with in ID8-mock tumors. In contrast, myeloid-derived suppressor cells (MDSCs) were significantly decreased in IL33-overexpressing tumors. Ascites from ID8-IL33 tumor-bearing mice inhibited MDSC differentiation. EOC patients with high staining of IL-33 had significantly longer overall survival times. We also established ID8 chemo-resistant subclones and heterogeneity assessment mouse models.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] PRIMA-1MET induces apoptosis through accumulation of intracellular reactive oxygen species irrespective of p53 status and chemo-sensitivity in epithelial ovarian cancer cells.2016
Author(s)
Yoshikawa N, Kajiyama H, Nakamura K, Utsumi F, Niimi K, Mitsui H, Sekiya R, Suzuki S, Shibata K, Callen D, Kikkawa F.
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Journal Title
Oncol Rep
Volume: 35(5)
Issue: 5
Pages: 2543-52
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The prognostic impact of pulmonary metastasectomy in recurrent gynecologic cancers: a retrospective single-institution study.2015
Author(s)
Adachi M, Mizuno M, Mitsui H, Kajiyama H, Suzuki S, Sekiya R, Utsumi F, Shibata K, Taniguchi T, Kawaguchi K, Yokoi K, Kikkawa F.
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Journal Title
Nagoya J Med Sci
Volume: 77(3)
Pages: 363-72
NAID
Related Report
Peer Reviewed
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[Journal Article] Opioid needs of terminally ill patients with gynecologic malignancies.2015
Author(s)
Utsumi F, Kajiyama H, Sakata J, Higashi M, Niimi K, Sekiya R, Mitsui H, Suzuki S, Umezu T, Mizuno M, Yamamoto E, Shibata K, Kikkawa F
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Journal Title
Int J Clin Oncol.
Volume: 20
Issue: 2
Pages: 405-10
DOI
Related Report
Peer Reviewed
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[Presentation] 抗癌剤耐性ヒト卵巣癌細胞株における5-アミノレブリン酸を用いた光線力学療法の検討2016
Author(s)
勅使河原 利哉, 水野 美香, 石井 琢也, 三井 寛子, 内海 史, 関谷 龍一郎, 鈴木 史朗, 梶山 広明, 柴田 清住, 高橋 究, 石塚 昌宏, 吉川 史隆
Organizer
第58回日本婦人科腫瘍学会学術講演会
Place of Presentation
米子コンベンションセンター(鳥取県米子市)
Year and Date
2016-07-08
Related Report
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