| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
When we administered metformin under ER stress induced by Tg, qPCR showed increased PlGF mRNA compared with thapsigargin only treatment and that this was dependent on metformin levels. Under ER stress, WB showed high levels of ATF4 and p-eIF2α but low levels of PlGF protein. By contrast, compared with thapsigargin alone, ATF4 and p-eIF2α levels were low and PlGF levels were high when metformin and thapsigargin were given, but these were again dependent on metformin concentrations. Western blot also confirmed that pravastatin attenuated ER stress and increased PlGF protein expression in trophoblast-like cells in a way similar to that observed for metformin.
CONCLUSION：Metformin and pravastatin suppressed ER stress via the PERK pathway and restored PlGF levels in trophoblast-like cells. These results indicate that metformin and pravastatin have a potential for preventing or treating PE.
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