| Project/Area Number |
15K20163
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 産科学 / lncRNA, placenta / lincRNA / expression |
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| Outline of Final Research Achievements |
[Purpose] H19 is maternally imprinted long non-coding RNA and also serves as a precursor for miR-675. Even though both are highly expressed in the placenta, expression cell types are still not reported. This time, we analyzed expression of H19 and miR-675-3p in mice term placenta.[Methods] Total RNA extracted from B6D2F1 placenta (E7.5~E18.5) was used for expression of H19 and miR-675. Expression positive cells were identified by in situ hybridization (ISH) using E18.5 placenta.[Results] By ISH at E18.5 labyrinth, H19 was expressed in fetal vascular endothelial cells and mononuclear trophoblasts, but not in syncytio trophoblasts. But miR-675-3p was positive in all placental barrier constituent cells. In addition, miR-675-3p was also positive in H19 negative spongy trophoblasts in the junctional zone.[Conclusion] This is the first report that miR-675-3p produced from H19 expressing cells was secreted to nearby H19 negative cells and need further investigation for detail functions.
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