Molecular biological research into anti-aging of voice using calorie restriction

• Project/Area Number: 15K20188
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: National Center for Global Health and Medicine
• Principal Investigator: Yamauchi Akihito 国立研究開発法人国立国際医療研究センター, その他部局等, 耳鼻咽喉科医師 (90612507)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 音声 / 老化 / アンチエイジング / カロリー制限 / 粘膜固有層 / 声帯瘢痕 / 長寿遺伝子 / 声帯

Outline of Final Research Achievements

The present study aimed at the invastigation of effects of short-term calorie restriction on the vocal fold lamina propria of young male rats as a basic research for anti-aging approaches toward age-related voice problems (presbyphonia).
Histological and immunohistological study revealed that rats with calorie restriction showed increased hyaluronic acid and sirt1, decreased collagen type III and oxydative stress, and ameliorated vocal fold scarring in response to the vocal fold injury. RT-PCR showed that calorie restricted rats demonstrated suppressed breakdown of hyaluronic acid, suppressed collagen production, and elevated sirt1.

Research Products

• [Journal Article] Characterization of Vocal Fold Vibration in Sulcus Vocalis Using High-Speed Digital Imaging. (2016)
  • Author(s): A. Yamauchi, H. Yokonishi, H. Imagawa, K.-I. Sakakibara, T. Nito, N. Tayama, T. Yamasoba
  • Journal Title: J. Speech Lang. Hear. Res. Volume: 60 Issue: 1 Pages: 24-37
  • DOI: 10.1044/2016_jslhr-s-14-0285
  • Peer Reviewed
• [Journal Article] 高速度デジタル撮像の評価法標準化にむけた取り組み (2016)
  • Author(s): 山内彰人、横西久幸、今川博、 榊原健一、高野真吾、二藤隆春、山岨達也、田山二朗
  • Journal Title: 喉頭 Volume: 28 Pages: 47-52
  • NAID: 130006792071
  • Peer Reviewed
• [Journal Article] 独立成分分析による混合筋電信号の分析方法の研究開発 (2016)
  • Author(s): 山内 彰人, 今川 博, 榊原 健一, 横西 久幸, 大塚 満美子, 後藤 多嘉緒, 二藤 隆春, 田山 二朗
  • Journal Title: 音声言語医学 Volume: 57 Pages: 227-237
  • NAID: 130005152341
  • Peer Reviewed
• [Journal Article] Quantitative analysis of vocal fold vibration in vocal fold paralysis with the use of high-speed digital imaging. (2015)
  • Author(s): Yamauchi A, Yokonishi H, Imagawa H, Sakakibara KI, Nito T, Tayama N, Yamasoba T.
  • Journal Title: J Voice[Accepted in October 2015] Volume: E-pub
  • Peer Reviewed
• [Journal Article] Visualization and estimation of vibratory disturbance in vocal fold scar using high-speed digital imaging. (2015)
  • Author(s): Yamauchi A, Yokonishi H, Imagawa H, Sakakibara KI, Nito T, Tayama N, Yamasoba T.
  • Journal Title: J Voice. 2015 [Epub ahead of print] Volume: E-pub
  • Peer Reviewed
• [Journal Article] Quantification of Vocal Fold Vibration in Various Laryngeal Disorders Using High-Speed Digital Imaging. (2015)
  • Author(s): Yamauchi A, Yokonishi H, Imagawa H, Sakakibara KI, Nito T, Tayama N, Yamasoba T.
  • Journal Title: J Voice. 2015 [Epub ahead of print] Volume: E-pub
  • Peer Reviewed
• [Journal Article] Vocal fold vibration in vocal fold atrophy: quantitative analysis with high-speed digital imaging. (2015)
  • Author(s): Yamauchi, A., Imagawa, H., Sakakibara, K-I., Yokonishi, H., Nito, T., Yamasoba, T., Tayama, N.
  • Journal Title: J Voice. 2015 [Epub ahead of print] Volume: E-pub
  • Peer Reviewed
• [Presentation] 高速度デジタル撮像から導出される声帯振動の音響学的特徴：健常者データについて (2017)
  • Author(s): 山内彰人 横西久幸 今川博 二藤隆春 榊原健一 田山二朗 山岨達也
  • Organizer: 第62回日本音声言語医学会
• [Presentation] 声帯開閉率(open quotient: OQ)と音質の関連における健常者の性差・年齢差 (2016)
  • Author(s): 山内彰人 横西久幸 今川博 二藤隆春 榊原健一 田山二朗 山岨達也
  • Organizer: 第61回日本音声言語医学会
  • Place of Presentation: 横浜
  • Year and Date: 2016-11-03
• [Presentation] 短期カロリー制限が声帯粘膜固有層に及ぼす影響について (2016)
  • Author(s): 山内彰人, 上羽瑠美, 藤巻葉子 近藤健二, 二藤隆春, 山岨達也
  • Organizer: 第16回日本抗加齢医学会
  • Place of Presentation: 横浜
  • Year and Date: 2016-06-10
• [Presentation] Quantification of vibrating area in normal and pathological voices using laryngotopography (2016)
  • Author(s): Akihito Yamauchi, Hiroshi Imagawa, Ken-Ichi Sakakibara, Hisayuki Yokonishi, Takaharu Nito, Niro Tayama, Tatsuya Yamasoba
  • Organizer: 10th East Asian Conference on Phonosurgery
  • Place of Presentation: 熊本、日本
  • Year and Date: 2016-03-04
  • Int'l Joint Research
• [Presentation] 高速度デジタル撮像の評価法標準化にむけた取り組み (2016)
  • Author(s): 山内彰人, 横西久幸, 今川博, 榊原健一, 高野真吾, 二藤隆春, 山岨達也, 田山二朗
  • Organizer: 第28回日本喉頭科学会
  • Place of Presentation: 大阪、日本
  • Year and Date: 2016-03-03
  • Invited
• [Presentation] 高速度デジタル撮像を用いた声帯振動の標準的評価法の確立に向けた取り組み (2016)
  • Author(s): 山内彰人, 横西久幸, 今川博, 榊原健一, 高野真吾, 二藤隆春, 山岨達也, 田山二朗
  • Organizer: 第26回気管食道科学会専門医大会
  • Place of Presentation: 東京、日本
  • Year and Date: 2016-02-20
  • Invited
• [Presentation] Quantitative analysis of vocal fold vibration in normal and pathological voices using high-speed digital imaging (2015)
  • Author(s): Akihito Yamauchi, Hiroshi Imagawa, Ken-Ichi Sakakibara, Hisayuki Yokonishi, Takaharu Nito, Niro Tayama
  • Organizer: 13th JTCOHNS
  • Place of Presentation: 東京、日本
  • Year and Date: 2015-12-03
  • Int'l Joint Research
• [Presentation] 新規パラメーターを用いた喉頭トポグラフィーによる声帯振動評価の改良 (2015)
  • Author(s): 山内彰人 横西久幸 今川博 後藤多嘉緒 上羽瑠美 二藤隆春 榊原健一 田山二朗 山岨達也
  • Organizer: 第60回日本音声医学会
  • Place of Presentation: 名古屋、日本
  • Year and Date: 2015-10-15
• [Presentation] Quantitative analysis of vocal fold vibration in normal and pathological voices using high-speed digital imaging (2015)
  • Author(s): Akihito Yamauchi, Hiroshi Imagawa, Ken-Ichi Sakakibara, Hisayuki Yokonishi, Takaharu Nito, Niro Tayama
  • Organizer: ＸＸＩＩＩ Pacific Voice Conference
  • Place of Presentation: サンノゼ、アメリカ
  • Year and Date: 2015-10-02
  • Int'l Joint Research / Invited
• [Presentation] LARYNGOTOPOGRAPHY FOR INTUITIVE EVALUATION OF SPATIAL CHARACTERISTICS OF VOCAL FOLD VIBRATION IN NORMAL AND PATHOLOGICAL VOICES (2015)
  • Author(s): Akihito Yamauchi, Hiroshi Imagawa, Hisayuki Yokonishi, Ken-Ichi Sakakibara, Takaharu Nito, Niro Tayama, Tatsuya Yamasoba,
  • Organizer: PEVOC11
  • Place of Presentation: イタリア、フィレンツェ
  • Year and Date: 2015-08-31
  • Int'l Joint Research
• [Book] “Quantitative Analysis of High-Speed Digital Imaging for the Elderly” Chapter in: K. Makiyama, and S Hirano (Eds.) (2017)
  • Author(s): Akihito Yamauchi
  • Total Pages: 115
  • Publisher: Springer
  • ISBN: 9811036977
• [Book] The principles. Normal and Abnormal Vocal Folds Kinematics Volume I Technology. (2015)
  • Author(s): Akihito Yamauchi, Hiroshi Imagawa, Ken-Ichi Sakakibara, Hisayuki Yokonishi, Takaharu Nito, Tatsuya Yamasoba, Niro Tayama
  • Total Pages: 4
  • Publisher: Pacific Voice & Speech Foundation.