Development and clinical application of new liquid biopsy method in nasopharyngeal carcinoma

• Project/Area Number: 15K20190
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Kanazawa University
• Principal Investigator: HIRAI NOBUYUKI 金沢大学, 附属病院, 医員 (40632534)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 上咽頭癌 / Epstein-Barr virus / miRNA / バイオマーカー / BART / biomarker / miR-BART17-5p / nasopharyngeal carcinoma

Outline of Final Research Achievements

Epstein-Barr virus (EBV) -related RNA in serum was quantified using serum from 31 patients with nasopharyngeal carcinoma. We examined three BamHI A rightward transcripts (BART) miRNAs among BART miRNAs which are EBV-related RNA this time. As a result of comparing the quantitative results with the stage and recurrence of nasopharyngeal carcinoma patients, it was found that miR-BART17-5p reflects the prognosis of patients with nasopharyngeal carcinoma after treatment most.

Research Products

• [Journal Article] Potential interest in circulating miR-BART17-5p as a post-treatment biomarker for prediction of recurrence in Epstein-Barr virus-related nasopharyngeal carcinoma (2016)
  • Author(s): Nobuyuki Hirai
  • Journal Title: PLOS ONE Volume: 11
  • NAID: 120005895691
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Post-treatment detection of circulating miR-BART17-5p as a potential biomarker to predict residual tumor cells in Epstein-Barr virus-related nasopharyngeal carcinoma (2016)
  • Author(s): Nobuyuki Hirai
  • Organizer: １７ｔｈ International Symposium on EBV and associated diseases
  • Place of Presentation: スイス
  • Year and Date: 2016-08-08
  • Int'l Joint Research
• [Presentation] Epstein-Barr virus関連上咽頭癌における治療後バイオマーカーとしてmiR-BART17-5pの可能性 (2016)
  • Author(s): 平井信行
  • Organizer: 第30回ヘルペスウイルス研究会
  • Place of Presentation: 東京
  • Year and Date: 2016-06-16
• [Presentation] Epstein-Barr virus関連上咽頭癌におけるバイオマーカーの検討 (2016)
  • Author(s): 平井信行
  • Organizer: 第117回日本耳鼻咽喉科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2016-05-18
• [Presentation] Epstein-Barr ウイルス関連上咽頭癌における新たなバイオマーカーの可能性 (2015)
  • Author(s): 平井信行
  • Organizer: 第28回 日本口腔・咽頭科学会
  • Place of Presentation: 大阪府大阪市 ホテル阪神
  • Year and Date: 2015-09-10
• [Presentation] Epstein-Barr ウイルス関連上咽頭癌における新たなバイオマーカーの可能性 (2015)
  • Author(s): 平井信行
  • Organizer: 第12回 EBV研究会
  • Place of Presentation: 島根県出雲市 ビックハート出雲
  • Year and Date: 2015-07-20