Association between activation induced cytidine deaminase and HPV positive oropharyngeal carcinogenesis

• Project/Area Number: 15K20192
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Kanazawa University
• Principal Investigator: Nakanishi Yosuke 金沢大学, 医学系, 助教 (20623648)
• Co-Investigator(Renkei-kenkyūsha): YOSHIZAKI Tomokazu 金沢大学, 医学系, 教授 (70262582)
• Research Collaborator: WAKISAKA Naohiro 金沢大学, 医学系, 准教授 (70377414) ; KONDO Satoru 金沢大学, 附属病院, 講師 (70436822) ; ENDO Kazuhira 金沢大学, 附属病院, 助教 (30547154)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 中咽頭癌 / 喉頭乳頭腫 / APOBEC family / APOBEC / HPV / human papilloma virus / ヒトパピローマウイルス

Outline of Final Research Achievements

We found that the expression of the apolipoprotein B mRNA-editing catalytic polypeptide (APOBEC) 3A, C, F, and H was higher in HPV positive oropharyngeal cancer (OPC) tissues than HPV negative OPC tissues. However, only the A3F was expressed in HPV positive laryngeal papilloma. We failed in conducting the transduction of the HPV gene into oropharyngeal cancer cell line because of low transduction efficiency.
We identified APOBEC family highly expressed in oropharyngeal cancer, It was suggested that laryngeal papilloma avoided the host immune response.

Research Products

• [Journal Article] Usefulness of human papillomavirus detection in oral rinse as a biomarker of oropharyngeal cancer (2017)
  • Author(s): Yoshida H, Murono S, Ueno T, Nakanishi Y, Tsuji A, Hatano M, Endo K, Kondo S, Sugimoto H, Wakisaka N, Yoshizaki T
  • Journal Title: Acta Otolaryngol Volume: 17 Pages: 1-5
  • Peer Reviewed
• [Journal Article] APOBEC3A associates with human papillomavirus genome integration in oropharyngeal cancers (2017)
  • Author(s): Kondo S, Wakae K, Wakisaka N, Nakanishi Y, Ishikawa K, Komori T, Moriyama-Kita M, Endo K, Murono S, Wang Z, Kitamura K, Nishiyama T, Yamaguchi K, Shigenobu S, Muramatsu M, Yoshizaki T
  • Journal Title: Oncogene Volume: 36 Pages: 1687-97
  • Peer Reviewed
• [Journal Article] EBV infection is prevalent in the adenoid and palatine tonsils in adults. (2017)
  • Author(s): Seishima N, Kondo S, Wakisaka N, Kobayashi E, Imoto T, Moriyama-Kita M, Nakanishi Y, Endo K, Murono S, Sugimoto H, Hatano M, Ueno T, Yoshizaki T.
  • Journal Title: J Med Virol Volume: 89 Pages: 1088-95
  • Peer Reviewed
• [Journal Article] Progression of understanding for the role of Epstein-Barr virus and management of nasopharyngeal carcinoma. (2017)
  • Author(s): Nakanishi Y, Wakisaka N, Kondo S, Endo K, Sugimoto H, Hatano M, Ueno T, Ishikawa K, Yoshizaki T.
  • Journal Title: Cancer Metastasis Rev Volume: 36 Pages: 435-447
  • Peer Reviewed
• [Presentation] 当科における局所進行喉頭癌の臨床検討 (2017)
  • Author(s): 中西庸介、吉崎智一
  • Organizer: 第29回日本喉頭科学会
• [Presentation] Lenvatinib therapy for advanced thyroid cancer (2017)
  • Author(s): Nakanishi Y, Yoshizaki T
  • Organizer: 2nd Congress of Asia Pacific Society of Thyroid Surgery
  • Int'l Joint Research
• [Presentation] 当科における局所進行喉頭癌の臨床検討 (2017)
  • Author(s): 中西庸介、吉崎 智一
  • Organizer: 第118回日本耳鼻咽喉科学会
• [Presentation] 早期声門癌における救済治療症例の検討 (2017)
  • Author(s): 中西庸介、室野重之、吉崎智一
  • Organizer: 第79回耳鼻咽喉科臨床学会
  • Int'l Joint Research
• [Presentation] Clicking larynxの2症例 (2017)
  • Author(s): 中西庸介、吉崎智一
  • Organizer: 第30回日本喉頭科学会