| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
I succeeded in generating in vivo and in vitro models for cholesteatoma tissue using keratinocytes and fibroblasts derived from mouse ear pinnae. With these models, I demonstrated that keratinocytes stimulate osteoclast differentiation through the induction of RANKL in fibroblasts. PGE2 significantly upregulated RANKL expression in fibroblasts , suggesting PGE2 is a candidate for a RANKL inducer.
Also in human cholesteatoma tissue, osteoclasts accumulated on the bone surface, and fibroblasts highly expressed RANKL.RNA sequence analysis of fibroblasts in perimatrix layer revealed highly expression of genes which involve in differentiation of osteoclasts.
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