Survival mechanism of CD271-positive HNSCC and itstherapeutic intervention

• Project/Area Number: 15K20238
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Miyagi Prefectural Hospital Organization Miyagi Cancer Center
• Principal Investigator: IMAI TAKAYUKI 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん先進治療開発研究部, 特任研究員 (80408583)
• Co-Investigator(Renkei-kenkyūsha): MATSUURA Kazuto 地方独立法人宮城県立病院機構宮城県立がんセンター(研究所), がん先進治療開発研究部, 特任研究員 (70271947)
• Research Collaborator: TANAKA Nobuyuki 地方独立法人宮城県立病院機構宮城県立がんセンター(研究所), がん先進治療開発研究部, 部長 (60280872)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: CD271 / 頭頸部癌 / 頭頚部外科学 / 癌幹細胞

Outline of Final Research Achievements

To determine a potential role of CD271 in a squamous cell carcinoma, CD271 was knock-downed by shRNA. CD271 mRNA knockdown inhibited both cell proliferation and migration. p42/44Erk inhibition as well as RhoA inhibition ameliorated the malignant phenotypes of hypopharyngeal HPCM2 cells. An anti-CD271 Ab, however, did not show a significant in vitro effect on the cells. Taken together, CD271 is shown to be a potential target for squamous cell carcinoma therapy.

Research Products

• [Journal Article] CD271 regulates the proliferation and motility of hypopharyngeal cancer cells (2016)
  • Author(s): Mochizuki M, Tamai K, Imai T, Sugawara S, Ogama N, Nakamura M, Matsuura K, Yamaguchi K, Satoh K, Sato I, Motohashi H, Sugamura K, Tanaka N
  • Journal Title: Sci Reｐ Volume: 6 Issue: 1 Pages: 30707-30707
  • DOI: 10.1038/srep30707
  • Peer Reviewed / Open Access
• [Journal Article] Clear cell carcinoma of the nasal cavity: A case report from histopathological viewpoint. (2016)
  • Author(s): Takayuki Imai, Ikuro Satoh, Ko Matsumoto, Shigemi Ito, Yukinori Asada, Kengo Kato, Yasutoshi Koshiba, Shigeru Saijo, Kazuto Matsuura
  • Journal Title: Auris Nasus Larynx Volume: 43(1) Issue: 1 Pages: 108-11
  • DOI: 10.1016/j.anl.2015.06.002
  • Peer Reviewed / Open Access
• [Presentation] 頭頸部癌においてCD271は増殖点浸潤を制御する (2016)
  • Author(s): 望月麻衣 玉井恵一 今井隆之 小鎌直子 中村真央 松浦一登 山口臺範 佐藤賢一 佐藤郁郎 菅村和夫 田中伸幸
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜市
  • Year and Date: 2016-12-02
• [Presentation] Functional Analysis of Hypopharyngeal CSCs marker CD271, focused on migration and invasion. (2015)
  • Author(s): Takayuki Imai, Mai Mochizuki
  • Organizer: 4th Congress of ASHNO, and 39th annual meeting of JSHNC
  • Place of Presentation: 神戸市
  • Year and Date: 2015-06-03
  • Int'l Joint Research