| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we aimed at the mechanism of expression and functional analysis of Ecel 1, which is expressed in the retina of glaucoma model mice. From the results of qPCR, western blotting and immunostaining, when retinal ganglion cell damage was induced by optic nerve crush, Ecel 1 was highly expressed in retinal ganglion cells 4 days after optic nerve crush. Although Ecel 1 is not induced by oxidative stress or NMDA injury, it is highly expressed by dysfunction of axonal transport as well as optic nerve crush. In order to analyze the function of Ecel1 in the retina, we made AAV2-mEcel1 virus vector which highly expresses Ecel1 in retinal ganglion cell. when this virus was administered in the eyes and optic nerve crush was carried out, unlike the hypothesis, no neuroprotective effect was observed in the group expressing high Ecel 1.
|
