The Role of Inverted Internal Limiting Membrane Flap in Macular Hole Closure

• Project/Area Number: 15K20262
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Okayama University
• Principal Investigator: SHIODE Yusuke 岡山大学, 大学病院, 助教 (20711097)
• Research Collaborator: 森實 祐基 ; 的場 亮 ; 平野 雅幸 ; 土居 真一郎 ; 戸島 慎二 ; 高橋 耕介 ; 荒木 亮一 ; 神﨑 勇希 ; 細木 三佳 ; 米澤 朋子 ; 吉田 篤史 ; 白神 史雄
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 黄斑円孔 / 内境界膜 / ミュラー細胞 / 神経栄養因子 / 内境界膜弁翻転法 / 細胞遊走

Outline of Final Research Achievements

We investigated the mechanism of macular hole (MH) closure following the inverted internal limiting membrane (ILM) technique. We performed the inverted ILM flap surgical technique as an experimental MH model in monkeys, and investigated the process of MH closure immunohistochemically. We then investigated the effects of type IV collagen, fibronectin, and laminin, which are constituent proteins of the ILM, on the proliferation and migration of cultivated Muller cells(MIO-M1). We also investigated the expression of neurotrophic factors in human ILM and MIO-M1 cells. From these results, during MH closure, the ILM functioned as a scaffold for the proliferation and migration of Muller cells, and may promote Muller cell activation. Neurotrophic factors produced by activated Muller cells and present on the surface of the ILM may contribute to MH closure.

Research Products

• [Journal Article] The Role of Inverted Internal Limiting Membrane Flap in Macular Hole Closure (2017)
  • Author(s): Shiode Yusuke、Morizane Yuki、Matoba Ryo、Hirano Masayuki、Doi Shinichiro、Toshima Shinji、Takahashi Kosuke、Araki Ryoichi、Kanzaki Yuki、Hosogi Mika、Yonezawa Tomoko、Yoshida Atsushi、Shiraga Fumio
  • Journal Title: Investigative Opthalmology & Visual Science Volume: 58 Issue: 11 Pages: 4847-4847
  • DOI: 10.1167/iovs.17-21756
  • Peer Reviewed / Open Access
• [Journal Article] A novel cell exclusion zone assay with a barrier made from room temperature vulcanizing silicone rubber (2017)
  • Author(s): Shiode Yusuke、Morizane Yuki、Matoba Ryo、Hirano Masayuki、Doi Shinichiro、Toshima Shinji、Araki Ryoichi、Hosogi Mika、Takahashi Kosuke、Kanzaki Yuki、Yonezawa Tomoko、Shiraga Fumio
  • Journal Title: PLOS ONE Volume: 12 Issue: 12 Pages: e0190198-e0190198
  • DOI: 10.1371/journal.pone.0190198
  • Peer Reviewed / Open Access
• [Journal Article] Surgical Outcome of Idiopathic Epiretinal Membranes with Intraretinal Cystic Spaces. (2016)
  • Author(s): Shiode Y, Morizane Y, Toshima S, Kimura S, Kumase F, Hosokawa M, Hirano M, Doi S, Takahashi K, Hosogi M, Fujiwara A, Shiraga F.
  • Journal Title: PLoS One. Volume: 12 Issue: 12 Pages: e0168555-e0168555
  • DOI: 10.1371/journal.pone.0168555
  • Peer Reviewed / Open Access
• [Journal Article] Efficacy of vitrectomy and inner limiting membrane peeling in age-related macular degeneration resistant to anti-vascular endothelial growth factor therapy, with vitreomacular traction or epiretinal membrane. (2016)
  • Author(s): Kimura S, Morizane Y, Toshima S, Hosogi M, Kumase F, Hosokawa M, Shiode Y, Fujiwara A, Shiraga F.
  • Journal Title: Graefes Arch Clin Exp Ophthalmol. Volume: --
  • Peer Reviewed
• [Journal Article] CLINICAL CHARACTERISTICS OF IDIOPATHIC FOVEOMACULAR RETINOSCHISIS. (2016)
  • Author(s): Maruko I, Morizane Y, Kimura S, Shiode Y, Hosokawa M, Sekiryu T, Iida T, Shiraga F.
  • Journal Title: Retina. Volume: --
  • Peer Reviewed
• [Journal Article] Successful displacement of a traumatic submacular hemorrhage in a 13-year-old boy treated by vitrectomy, subretinal injection of tissue plasminogen activator and intravitreal air tamponade: a case report. (2015)
  • Author(s): Doi S, Kimura S, Morizane Y, Shiode Y, Hosokawa M, Hirano M, Hosogi M, Fujiwara A, Miyamoto K, Shiraga F.
  • Journal Title: BMC Ophthalmol. Volume: -- Issue: 1 Pages: 94-94
  • DOI: 10.1186/s12886-015-0090-3
  • Peer Reviewed / Open Access
• [Journal Article] Planned foveal detachment technique for the resolution of diffuse diabetic macular edema. (2015)
  • Author(s): Morizane Y, Kimura S, Hosokawa M, Shiode Y, Hirano M, Doi S, Hosogi M, Fujiwara A, Inoue Y, Shiraga F.
  • Journal Title: Jpn J Ophthalmol. Volume: 5 Issue: 5 Pages: 279-287
  • DOI: 10.1007/s10384-015-0390-4
  • Peer Reviewed
• [Journal Article] Case report: successful closure of a large macular hole secondary to uveitis using the inverted internal limiting membrane flap technique. (2015)
  • Author(s): Hirano M, Morizane Y, Kawata T, Kimura S, Hosokawa M, Shiode Y, Doi S, Hosogi M, Fujiwara A, Shiraga F.
  • Journal Title: BMC Ophthalmol. Volume: -- Issue: 1
  • DOI: 10.1186/s12886-015-0072-5
  • Peer Reviewed / Open Access
• [Journal Article] COMPARISON OF HALVING THE IRRADIATION TIME OR THE VERTEPORFIN DOSE IN PHOTODYNAMIC THERAPY FOR CHRONIC CENTRAL SEROUS CHORIORETINOPATHY. (2015)
  • Author(s): Shiode Y, Morizane Y, Kimura S, Hosokawa M, Kawata T, Doi S, Hosogi M, Fujiwara A, Shiraga F.
  • Journal Title: Retina. Volume: 12 Issue: 12 Pages: 2498-2504
  • DOI: 10.1097/iae.0000000000000621
  • Peer Reviewed
• [Journal Article] Six-month results of intravitreal aflibercept injections for patients with polypoidal choroidal vasculopathy. (2015)
  • Author(s): Hosokawa M, Shiraga F, Yamashita A, Shiragami C, Ono A, Shirakata Y, Kimura S, Shiode Y, Kawata T, Hosogi M, Fujiwara A, Morizane Y.
  • Journal Title: Br J Ophthalmol. Volume: 8 Issue: 8 Pages: 1087-1091
  • DOI: 10.1136/bjophthalmol-2014-305275
  • Peer Reviewed
• [Journal Article] Submacular hemorrhage in polypoidal choroidal vasculopathy treated by vitrectomy and subretinal tissue plasminogen activator. (2015)
  • Author(s): Kimura S, Morizane Y, Hosokawa M, Shiode Y, Kawata T, Doi S, Matoba R, Hosogi M, Fujiwara A, Inoue Y, Shiraga F.
  • Journal Title: Am J Ophthalmol. Volume: 4 Issue: 4 Pages: 683-689
  • DOI: 10.1016/j.ajo.2014.12.020
  • Peer Reviewed
• [Presentation] 細胞外基質が細胞遊走に及ぼす影響の評価を目的とした細胞遊走アッセイ (2018)
  • Author(s): 塩出雄亮，森實祐基，平野雅幸，土居真一郎，戸島慎二，荒木亮一，高橋耕介，松前洋，神﨑勇希，細木三佳，米澤朋子，白神史雄
  • Organizer: 第122回 日本眼科学会総会
• [Presentation] 中心性漿液性脈絡網膜症に対する光線力学療法が脈絡膜の厚みと血管密度に及ぼす影響 (2016)
  • Author(s): 塩出雄亮、森實祐基、木村修平、熊瀬文明、細川海音、土居真一郎、平野雅幸、戸島慎二、高橋耕介、細木三佳、藤原篤之、白神史雄
  • Organizer: 第55回日本網膜硝子体学会
  • Place of Presentation: 東京
  • Year and Date: 2016-12-02
• [Presentation] 特発性黄斑上膜にみられる網膜内嚢胞の検討 (2016)
  • Author(s): 杉原佳恵、塩出雄亮、森實祐基、戸島慎二、木村修平、熊瀬文明、細川海音、平野雅幸、土居真一郎、細木三佳、藤原篤之、白神史雄
  • Organizer: 第70回日本臨床眼科学会
  • Place of Presentation: 京都
  • Year and Date: 2016-11-03
• [Presentation] 特発性黄斑上膜にみられる嚢胞様黄斑浮腫の検討 (2015)
  • Author(s): 塩出雄亮、森實祐基、平野雅幸、木村修平、熊瀬文明、細川海音、土居真一郎、戸島慎二、細木三佳、藤原篤史、白神史雄
  • Organizer: 第54回日本網膜硝子体学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2015-12-04
• [Presentation] 中心性漿液性脈絡網膜症の治療前後の脈絡膜厚および脈絡膜血管密度の検討 (2015)
  • Author(s): 塩出雄亮、森實祐基、木村修平、熊瀬文明、細川海音、土居真一郎、平野雅幸、戸島慎二、細木三佳、藤原篤史、白神史雄
  • Organizer: 第69回日本臨床眼科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-22
• [Presentation] Muller細胞の増殖、遊走におけるⅣ型コラーゲンの役割 (2015)
  • Author(s): 塩出雄亮、森實祐基、的場亮、平野雅幸、土居真一郎、荒木亮一、米澤朋子、白神史雄
  • Organizer: 第119回日本眼科学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2015-04-16
• [Book] 眼科疾患最新の治療2016-2018 (2016)
  • Author(s): 大橋裕一/白神史雄/村上晶
  • Total Pages: 344
  • Publisher: 南江堂
• [Book] OCT便利手帖 (2015)
  • Author(s): 白神 史雄/飯田 知弘/石龍 鉄樹
  • Total Pages: 173
  • Publisher: メジカルビュー社