Pathology of age-related macular degeneration using polarized RPE cells

• Project/Area Number: 15K20270
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Kagoshima University
• Principal Investigator: Terasaki Hiroto 鹿児島大学, 医歯学総合研究科, 客員研究員 (20746888)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 加齢黄斑変性 / 網膜色素上皮細胞 / 血管内皮細胞増殖因子

Outline of Final Research Achievements

Effects of anti-vascular endothelial growth factor(VEGF) on polarized RPE cells were studied. All of these drugs did not affect cellular toxicity or barrier function in polarized RPE cells. However, we found that there were differences of anti-VEGF effect and permeability between drugs in polarized RPE cells. These results suggested that we might be able to clarify the choice of anti-VEGF drugs based upon clinical findings.

Research Products

• [Journal Article] CHOROIDAL STRUCTURE ALTERED BY DEGENERATION OF RETINA IN EYES WITH RETINITIS PIGMENTOSA. (2017)
  • Author(s): Kawano H, Sonoda S, Saito S, Terasaki H, Sakamoto T.
  • Journal Title: Retina Volume: - Issue: 11 Pages: 2175-2182
  • DOI: 10.1097/iae.0000000000001465
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] PERMEABILITY AND ANTI-VASCULAR ENDOTHELIAL GROWTH FACTOR EFFECTS OF BEVACIZUMAB, RANIBIZUMAB, AND AFLIBERCEPT IN POLARIZED RETINAL PIGMENT EPITHELIAL LAYER IN VITRO. (2017)
  • Author(s): Yoshihara N, Terasaki H, Shirasawa M, Kawano H, Sonoda S, Yamakuchi M, Hashiguchi T, Hisatomi T, Ishibashi T, Sakamoto T.
  • Journal Title: Retina Volume: 37(1) Issue: 1 Pages: 179-190
  • DOI: 10.1097/iae.0000000000001117
  • Peer Reviewed
• [Journal Article] Correlations Between Retinal Nerve Fiber Layer Thickness and Axial Length, Peripapillary Retinal Tilt, Optic Disc Size, and Retinal Artery Position in Healthy Eyes. (2017)
  • Author(s): Yamashita T, Sakamoto T, Yoshihara N, Terasaki H, Tanaka M, Kii Y, Nakao K.
  • Journal Title: J Glaucoma. Volume: 26 Issue: 1 Pages: 34-40
  • DOI: 10.1097/ijg.0000000000000550
  • Peer Reviewed / Open Access
• [Journal Article] Location of Tessellations in Ocular Fundus and Their Associations with Optic Disc Tilt, Optic Disc Area, and Axial Length in Young Healthy Eyes. (2016)
  • Author(s): Terasaki H, Yamashita T, Yoshihara N, Kii Y, Tanaka M, Nakao K, Sakamoto T.
  • Journal Title: PLoS One. Volume: 11 Issue: 6 Pages: e0156842-e0156842
  • DOI: 10.1371/journal.pone.0156842
  • Peer Reviewed / Open Access
• [Journal Article] Minification of fundus optical coherence tomographic images in gas-filled eye. (2016)
  • Author(s): Yamashita T, Terasaki H, Sakamoto T.
  • Journal Title: BMC Ophthalmol. Volume: 16 Issue: 1
  • DOI: 10.1186/s12886-016-0306-1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Changes in visual field defects during 10-year follow-up for indocyanine green-assisted macular hole surgery. (2016)
  • Author(s): Nakazawa M, Terasaki H, Yamashita T, Uemura A, Sakamoto T.
  • Journal Title: Jpn J Ophthalmol. Volume: 60 Issue: 5 Pages: 383-387
  • DOI: 10.1007/s10384-016-0453-1
  • Peer Reviewed
• [Journal Article] Humanin Protects RPE Cells from Endoplasmic Reticulum Stress-Induced Apoptosis by Upregulation of Mitochondrial Glutathione. (2016)
  • Author(s): Matsunaga D, Sreekumar PG, Ishikawa K, Terasaki H, Barron E, Cohen P, Kannan R, Hinton DR.
  • Journal Title: PLoS ONE Volume: 11 Issue: 10 Pages: e0165150-e0165150
  • DOI: 10.1371/journal.pone.0165150
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] AMOUNT OF RESIDUAL SILICONE OIL IN VITREOUS CAVITY IS SIGNIFICANTLY CORRELATED WITH AXIAL LENGTH. (2016)
  • Author(s): Shiihara H, Terasaki H, Yoshihara N, Shirasawa M, Otuka H, Yamashit T, Yamakiri K, Sonoda S, Sakamoto T.
  • Journal Title: Retina Volume: 36 Issue: 1 Pages: 181-187
  • DOI: 10.1097/iae.0000000000000656
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Penetration of bevacizumab and ranibizumab through retinal pigment epithelial layer in vitro. (2015)
  • Author(s): Terasaki H, Sakamoto T, Shirasawa M, Yoshihara N, Otsuka H, Sonoda S, Hisatomi T, Ishibashi T.
  • Journal Title: Retina Volume: 35 Issue: 5 Pages: 1007-1015
  • DOI: 10.1097/iae.0000000000000428
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Non-rhegmatogenous retinal detachment associated with hair-like object in vitreous: A case report (2015)
  • Author(s): Terasaki H, Nakao K, Kazuki F, Hiraki T, Shirasawa W, Abematsu N, Yonezawa S, Sakamoto T
  • Journal Title: Clin Exp Optom Volume: 98 Issue: 4 Pages: 379-380
  • DOI: 10.1111/cxo.12280
  • Peer Reviewed / Open Access
• [Journal Article] Luminal and stromal areas of choroid determined by binarization method of optical coherence tomographic images. (2015)
  • Author(s): Sonoda S, Sakamoto T, Yamashita T, Uchino E, Kawano H, Yoshihara N, Terasaki H, Shirasawa M, Tomita M, Ishibashi T
  • Journal Title: Am J Ophthalmol Volume: NA Issue: 6 Pages: 1123-1131
  • DOI: 10.1016/j.ajo.2015.03.005
  • Peer Reviewed / Open Access
• [Journal Article] Circumpapillary course of retinal pigment epithelium can be fit to sine wave and amplitude of sine wave is significantly correlated with ovality ratio of optic disc. (2015)
  • Author(s): Yamashita T, Sakamoto T, Yoshihara N, Terasaki H, Kii Y, Tanaka M, Nakao K.
  • Journal Title: PLoS One Volume: 10 Issue: 4 Pages: e0122191-e0122191
  • DOI: 10.1371/journal.pone.0122191
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Different Effects of Thrombin on VEGF Secretion, Proliferation, and Permeability in Polarized and Non-polarized Retinal Pigment Epithelial Cells (2015)
  • Author(s): Terasaki H, Shirasawa M, Otsuka H, Yamashita T, Uchino E, Hisatomi T, Sonoda S, Sakamoto T
  • Journal Title: Current Eye Research Volume: 40 Issue: 9 Pages: 936-945
  • DOI: 10.3109/02713683.2014.964417
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Resveratrol inhibits epithelial-mesenchymal transition of retinal pigment epithelium and development of proliferative vitreoretinopathy (2015)
  • Author(s): Ishikawa K, He S, Terasaki H,Nazari H, Zhang H, Spee C, Kannan R, Hinton DR
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 16386-16386
  • DOI: 10.1038/srep16386
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Increased expression and apical distribution of βA3/A1-Crystallin in polarized RPE cells (2016)
  • Author(s): Hiroto Terasaki, Parameswaran G. Sreekumar, Shozo Sonoda, Christine Spee, Taiji Sakamoto, David R. Hinton, Ram Kannan
  • Organizer: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: ワシントン州シアトル（米国）
  • Year and Date: 2016-05-03
  • Int'l Joint Research
• [Presentation] 抗VEGF薬が極性網膜色素上皮細胞に 与える影響と薬剤による違い (2015)
  • Author(s): 寺崎寛人
  • Organizer: 第85回九州眼科学会
  • Place of Presentation: 鹿児島県鹿児島市鹿児島県医師会館
  • Year and Date: 2015-05-29
• [Presentation] The effect of aflibercept on the barrier function of polarized retinal pigment epithelium cells in vitro (2015)
  • Author(s): Terasaki H, Yoshihara N, Shirasawa M, Otsuka H, Sonoda S, Sakamoto T
  • Organizer: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: 米国コロラド州デンバー
  • Year and Date: 2015-05-03
  • Int'l Joint Research
• [Presentation] 極性網膜色素上皮細胞を用いた 加齢黄斑変性の病態と 抗VEGF薬の作用機序の解明 (2015)
  • Author(s): 寺崎寛人
  • Organizer: 第119回日本眼科学会総会
  • Place of Presentation: 北海道札幌市さっぽろ芸文館 ニトリ文化ホール
  • Year and Date: 2015-04-16