| Project/Area Number |
15K20285
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Keio University |
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Principal Investigator |
Niwano Hiroko 慶應義塾大学, 医学部(信濃町), 研究員 (90748518)
|
|---|
| Research Collaborator |
SHIMMURA SHIGETO
MIYASHITA HIDEYUKI
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| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 角膜上皮 / 幹細胞 / 培養 |
|---|
| Outline of Final Research Achievements |
To confirm whether the cultured epithelial sheets contain slow cycling cells as like in vivo tissue, the researcher labeled proliferating cells in limbal epithelial cells around confluence by serial 3 day feeding of EdU, and cultured confluent sheets for additional 6 months. Although EdU rate was significantly decreased from 58.8% to 8.8%, EdU rate in undifferentiated epithelial cells (K15+ cell clusters) was 34.8%, was not significantly different from the rete 6.0% in K15 negative differentiated cells (p=0.09), due to its high variance. We published our data including this study in Scientific Reports on 2017, 10.1038/srep43557.
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