Anti-tumor effect of Pyrrole-Imidazole Polyamide(PIP) targeting angiogenic system in neuroblastoma
| Project/Area Number |
15K20302
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatric surgery
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| Research Institution | Nihon University |
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Principal Investigator |
UEKUSA Shota 日本大学, 医学部, 専修医 (70746338)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 神経芽腫 / 機能解析 / 細胞浸潤能 / Neuropilin1 / Integrinβ / NRP1 / MMP9 |
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| Outline of Final Research Achievements |
Although it has been known that neuropilin1 (NRP1) has a promoting effect in vascularization, which is critical process in growth and progression of malignant tumors, the function of NRP1 in neuroblastoma cells is still unclear. In the present study, we conducted functional analyses of NRP1 to elucidate its roles in the development and/or promotion of neuroblastoma. In addition, we synthesized pyrrole imidazole polyamides (PIP) targeting to NRP1, and investigated its potential as therapeutic agents for neuroblastoma. Our current result demonstrated that the PIPs we designed did not suppress the expression of NRP1 in neuroblastoma cells. SiRNA mediated silencing of NRP1 in neuroblastoma derived SK-N-AS cells resulted in up-regulation of Integrin b1, and promotion of cell migration and invasion.
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Report
(3 results)
Research Products
(5 results)
