Visualizing fetal skin wound tissue by whole-mount immunohistochemistry.

• Project/Area Number: 15K20323
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Keio University
• Principal Investigator: Sakio Reiko 慶應義塾大学, 医学部(信濃町), 助教 (70723261)
• Research Collaborator: KISHI Kazuo
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 創傷治癒 / 再生 / 血管新生

Outline of Final Research Achievements

To explore the mechanism of scarless wound healing, fetal skin wounds were created and analyzed using whole-mount immunohistochemistry. In the wound tissue of E13.5 embryos, numerous endothelial cells were observed extending their filopodia towards the center of the wounds, whereas after E15.5 seldom endothelial cells were observed in the superficial layer of the wounds. The surface of E13.5 wounds were covered with numerous macrophages, which were no longer observed after E15.5. These features may be related to the potential of skin wound to heal without scarring. Further morphological analysis in conjunction with genetic modification of animals will elucidate new insights into the mechanism of skin wound healing.