| Budget Amount *help |
¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Outline of Final Research Achievements |
DAI is a severe TBI,associated with poor outcome whose mortality exceeds 40%.However,diagnostics of DAI has not yet been established,especially in the early phase after injury.We hypothesized that serum level of tau protein,which was localized in neuronal axons, would be a useful biomarker for diagnosis and severity assessment of DAI.To elucidate relationship between severity of DAI and serum tau protein concentration,using an animal model. We evaluated association between serum concentration of tau and severity of DAI using a rat model of DAI.DAI was induced by dropping a weight in the head from a predetermined height(mild DAI group;1m,severe DAI group;2m).Eighteen rats were divided into 3 groups,sham-operated(n=4),mild DAI(n=7)and severe DAI(n=7).Serum levels of tau protein were measured by enzyme linked-immunosorbent assay.The serum levels of tau at 60 minutes after injury were statistically different among three groups(sham<mild<severe,sham vs.mild,p=0.024;mild vs.severe,p=0.018)
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