Project/Area Number |
15K20340
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Emergency medicine
|
Research Institution | Osaka University |
Principal Investigator |
Yamada Tomoki 大阪大学, 医学系研究科, 特任研究員 (40623434)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | 腸管上皮幹細胞 / 腸管機能不全 / 全身性炎症症候群 / 敗血症 / Lgr5 / Wnt / 全身性炎症反応症候群 / BrdU |
Outline of Final Research Achievements |
The mechanism of intestinal dysfunction develops during systemic invasion has not been sufficiently elucidated. We focused on the function of intestinal stem cells and aimed to elucidate this mechanism. First, in the CLP model, the number of cells during cell division in the crypt was decreasing. The signals involved in the proliferation or differentiation of intestinal stem cells were evaluated by RT-PCR, and it was found that Wnt3, BMP4 (that inhibits Wnt), Noggin (that inhibits BMP), and Notch1 (that is involved in maintaining undifferentiated state of intestinal stem cell) were decreased after 24 hours in the CLP group. The number of Lgr5 positive cells was also decreased in the CLP group. Intestinal stem cell damage caused by CLP may cause villous atrophy and intestinal peristaltic dysfunction.
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