Analysis on the supply system of phosphate ions by osteoblasts/osteocytes during bone mineralization
| Project/Area Number |
15K20356
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Research Collaborator |
KOMORI Toshihumi 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (00252677)
ODA Kimimitsu 新潟大学, 医歯学系, 教授 (10122681)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 骨基質石灰化 / 骨芽細胞 / TNALP / 石灰化 / 骨細胞 / ENPP1 |
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| Outline of Final Research Achievements |
In order to elucidate the regulation of phosphate ion generation by chondrocyte and osteoblastic cells (preosteoblast, osteoblast and osteocyte) during the process of bone mineralization, we generated transgenic mice over-expressing tissue non-specific alkaline phosphatase (TNALP) driven by type I collagen promoter specific to preosteoblast/osteoblast, or type II collagen promoter specific to chondrocyte. We have examined the localization of several enzymes and membrane transporters involved in phosphate ion generation and mineralization in bone.
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Report
(3 results)
Research Products
(18 results)
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[Journal Article] Biological application of focus ion beam-scanning electron microscopy (FIB-SEM) to the imaging of cartilaginous fibrils and osteoblastic cytoplasmic processes.2017
Author(s)
Hasegawa T., Endo T., Tsuchiya E., Kudo A., Zhao S., Moritani Y., Abe M.,Yamamoto T., Hongo H., Tsuboi K., Yoshida T., Nagai T., Khadiza N., Yokoyama A., Freitas de PHL., Li M., Amizuka N.
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Journal Title
J Oral Biosci.
Volume: 59
Issue: 1
Pages: 55-62
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Histochemical examination on periodontal tissues of Klotho-deficient mice fed with phosphate insufficient diet.2017
Author(s)
Hikone K., Hasegawa T., Tsuchiya E., Hongo H., Sasaki M., Yamamoto T., Kudo A., Oda K., Haraguchi M., Freitas PHL., Li M., Iida J., Amizuka N.
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Journal Title
The Journal of Histochemistry and Cytochemistry
Volume: 65巻4号
Issue: 4
Pages: 207-221
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Ultrastructural and biochemical aspects of matrix vesicle-mediated mineralization.2017
Author(s)
Hasegawa T., Yamamoto T., Tsuchiya E., Hongo H., Tsuboi K., Kudo A., Abe M., Yoshida T., Nagai T., Khadiza N., Yokoyama A., Oda K., Ozawa H., Freitas de PHL., Li M., Amizuka N.
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Journal Title
Japanese Dental Science Review.
Volume: 印刷中
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Treatment with eldecalcitol positively affects mineralization, microdamage, and collagen crosslinks in primate bone.2015
Author(s)
Saito M, Grynpas MD, Burr DB, Allen MR, Smith SY, Doyle N, Amizuka N, Hasegawa T, Kida Y, Marumo K, Saito H.
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Journal Title
Bone.
Volume: 73
Pages: 8-15
DOI
Related Report
Peer Reviewed / Open Access
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