| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In this study, we tried virulence analysis of surface proteins on periodontal pathogen Aggregatibacter actinomycetemcomitans. To analysis the exposed proteins on outer membrane, trypsin shaving was performed. As the results, several surface proteins including previously known virulence factors Omp100 and EmaA were identified. However, many of the identified proteins were intracellular located proteins such as the ribosomal proteins, chaperons and enzymes. These results indicate some of the identified intracellular proteins are derivative from killed bacteria by trypsin treatment. Improved protocol is needed to systematic identification of surface proteins in this microbe.
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