| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The salivary gland rudiments in embryonic day 12 were separated into the epithelium of submandibular gland and oral epithelium far from the salivary gland by laser micro-dissection, and performed analysis of differential gene expression by RNA-seq. We identified the two transcriptional factors that regulate specification of salivary gland rudiment at the earliest stages of salivary gland development. To determine whether the transcriptional factors promote salivary gland differentiation, mouse embryonic stem cells-derived oral ectoderm was transduced with Ad-vector expressing transcription factors. During days 23, we observed the aggregates underwent a dynamic shape change and formed branching morphology. The ESCs-derived exocrine gland structures had three-dimensional salivary gland structures. Moreover, orthotopic transplantation of ESCs-derived salivary gland could replace salivary gland in a mouse model of a parotid gland defect.
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